2alz

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[[Image:2alz.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2alz| PDB=2alz | SCENE= }}
{{STRUCTURE_2alz| PDB=2alz | SCENE= }}
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'''Ternary Complex of hPoli with DNA and dCTP'''
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===Ternary Complex of hPoli with DNA and dCTP===
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==Overview==
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Human DNA polymerase iota (hPoliota), a member of the Y family of DNA polymerases, differs in remarkable ways from other DNA polymerases, incorporating correct nucleotides opposite template purines with a much higher efficiency and fidelity than opposite template pyrimidines. We present here the crystal structure of hPoliota bound to template G and incoming dCTP, which reveals a G.C + Hoogsteen base pair in a DNA polymerase active site. We show that the hPoliota active site has evolved to favor Hoogsteen base pairing, wherein the template sugar is fixed in a cavity that reduces the C1'-C1' distance across the nascent base pair from approximately 10.5 A in other DNA polymerases to 8.6 A in hPoliota. The rotation of G from anti to syn is then largely in response to this curtailed C1'-C1' distance. A G.C+ Hoogsteen base pair suggests a specific mechanism for hPoliota's ability to bypass N(2)-adducted guanines that obstruct replication.
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(as it appears on PubMed at http://www.pubmed.gov), where 16216587 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16216587}}
==About this Structure==
==About this Structure==
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[[Category: Template g. incoming dctp]]
[[Category: Template g. incoming dctp]]
[[Category: Ternary complex]]
[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:12:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:26:47 2008''

Revision as of 14:26, 27 July 2008

Image:2alz.png

Template:STRUCTURE 2alz

Ternary Complex of hPoli with DNA and dCTP

Template:ABSTRACT PUBMED 16216587

About this Structure

2ALZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2005 Oct;13(10):1569-77. PMID:16216587

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