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1s3t

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[[Image:1s3t.gif|left|200px]]
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{{STRUCTURE_1s3t| PDB=1s3t | SCENE= }}
{{STRUCTURE_1s3t| PDB=1s3t | SCENE= }}
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'''BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE'''
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===BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE===
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==Overview==
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The structure of the complex of urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 A resolution. The complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the B(OH)3 molecule bridging the Ni ions and leaving in place the bridging hydroxide. Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis.
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{{ABSTRACT_PUBMED_15038715}}
==About this Structure==
==About this Structure==
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[[Category: Nickel]]
[[Category: Nickel]]
[[Category: Urease]]
[[Category: Urease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:15:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:28:59 2008''

Revision as of 14:29, 27 July 2008

Image:1s3t.png

Template:STRUCTURE 1s3t

BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE

Template:ABSTRACT PUBMED 15038715

About this Structure

1S3T is a Protein complex structure of sequences from Sporosarcina pasteurii. Full crystallographic information is available from OCA.

Reference

Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism., Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S, J Am Chem Soc. 2004 Mar 31;126(12):3714-5. PMID:15038715

Page seeded by OCA on Sun Jul 27 17:28:59 2008

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