1mqv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1mqv.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1mqv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1mqv| PDB=1mqv | SCENE= }}
{{STRUCTURE_1mqv| PDB=1mqv | SCENE= }}
-
'''Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli'''
+
===Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli===
-
==Overview==
+
<!--
-
We employed fluorescence energy-transfer probes to investigate the polypeptide dynamics accompanying cytochrome c' folding. Analysis of fluorescence energy-transfer kinetics from wild-type Trp-72 or Trp-32 in a crystallographically characterized (1.78 A) Q1A/F32W/W72F mutant shows that there is structural heterogeneity in denatured cytochrome c'. Even at guanidine hydrochloride concentrations well beyond the unfolding transition, a substantial fraction of the polypeptides ( approximately 50%) adopts compact conformations (tryptophan-to-heme distance, approximately 25 A) in both pseudo-wild-type (Q1A) and mutant proteins. A burst phase (&lt; or =5 ms) is revealed when stopped flow-triggered refolding is probed by tryptophan intensity: measurements on the Q1A protein show that approximately 75% of the Trp-72 fluorescence (83% for Trp-32) is quenched within the mixing deadtime, suggesting that most of the polypeptides have collapsed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12407175}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12407175 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12407175}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Winkler, J R.]]
[[Category: Winkler, J R.]]
[[Category: Four-helix bundle]]
[[Category: Four-helix bundle]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:36:44 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:33:37 2008''

Revision as of 14:33, 27 July 2008

Image:1mqv.png

Template:STRUCTURE 1mqv

Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli

Template:ABSTRACT PUBMED 12407175

About this Structure

1MQV is a Single protein structure of sequence from Rhodopseudomonas palustris. Full crystallographic information is available from OCA.

Reference

Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics., Lee JC, Engman KC, Tezcan FA, Gray HB, Winkler JR, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14778-82. Epub 2002 Oct 29. PMID:12407175

Page seeded by OCA on Sun Jul 27 17:33:37 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mqv"
Personal tools