1v49

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[[Image:1v49.gif|left|200px]]
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{{STRUCTURE_1v49| PDB=1v49 | SCENE= }}
{{STRUCTURE_1v49| PDB=1v49 | SCENE= }}
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'''Solution structure of microtubule-associated protein light chain-3'''
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===Solution structure of microtubule-associated protein light chain-3===
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==Overview==
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Microtubule-associated protein (MAP) light chain 3 (LC3) is a human homologue of yeast Apg8/Aut7/Cvt5 (Atg8), which is essential for autophagy. MAP-LC3 is cleaved by a cysteine protease to produce LC3-I, which is located in cytosolic fraction. LC3-I, in turn, is converted to LC3-II through the actions of E1- and E2-like enzymes. LC3-II is covalently attached to phosphatidylethanolamine on its C terminus, and it binds tightly to autophagosome membranes. We determined the solution structure of LC3-I and found that it is divided into N- and C-terminal subdomains. Additional analysis using a photochemically induced dynamic nuclear polarization technique also showed that the N-terminal subdomain of LC3-I makes contact with the surface of the C-terminal subdomain and that LC3-I adopts a single compact conformation in solution. Moreover, the addition of dodecylphosphocholine into the LC3-I solution induced chemical shift perturbations primarily in the C-terminal subdomain, which implies that the two subdomains have different sensitivities to dodecylphosphocholine micelles. On the other hand, deletion of the N-terminal subdomain abolished binding of tubulin and microtubules. Thus, we showed that two subdomains of the LC3-I structure have distinct functions, suggesting that MAP-LC3 can act as an adaptor protein between microtubules and autophagosomes.
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{{ABSTRACT_PUBMED_15857831}}
==About this Structure==
==About this Structure==
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1V49 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V49 OCA].
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1V49 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V49 OCA].
==Reference==
==Reference==
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[[Category: Ueno, T.]]
[[Category: Ueno, T.]]
[[Category: Ubiquitin fold]]
[[Category: Ubiquitin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:03:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:34:29 2008''

Revision as of 14:34, 27 July 2008

Image:1v49.png

Template:STRUCTURE 1v49

Solution structure of microtubule-associated protein light chain-3

Template:ABSTRACT PUBMED 15857831

About this Structure

1V49 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains., Kouno T, Mizuguchi M, Tanida I, Ueno T, Kanematsu T, Mori Y, Shinoda H, Hirata M, Kominami E, Kawano K, J Biol Chem. 2005 Jul 1;280(26):24610-7. Epub 2005 Apr 27. PMID:15857831

Page seeded by OCA on Sun Jul 27 17:34:29 2008

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