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2pex

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[[Image:2pex.jpg|left|200px]]
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{{STRUCTURE_2pex| PDB=2pex | SCENE= }}
{{STRUCTURE_2pex| PDB=2pex | SCENE= }}
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'''Structure of reduced C22S OhrR from Xanthamonas Campestris'''
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===Structure of reduced C22S OhrR from Xanthamonas Campestris===
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==Overview==
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The Xanthomonas campestris transcription regulator OhrR contains a reactive cysteine residue (C22) that upon oxidation by organic hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue C127'. Such modification induces the expression of a peroxidase that reduces OHPs to their less toxic alcohols. Here, we describe the structures of reduced and OHP-oxidized OhrR, visualizing the structural mechanism of OHP induction. Reduced OhrR takes a canonical MarR family fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the disruption of the Y36'-C22-Y47' interaction network and dissection of helix alpha5, which then allows the 135 degrees rotation and 8.2 A translation of C127', formation of the C22-C127' disulphide bond, and alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These changes result in the 28 degrees rigid body rotations of each winged helix-turn-helix motif and DNA dissociation. Similar effector-induced rigid body rotations are expected for most MarR family members.
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(as it appears on PubMed at http://www.pubmed.gov), where 18042459 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18042459}}
==About this Structure==
==About this Structure==
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[[Category: Newberry, K J.]]
[[Category: Newberry, K J.]]
[[Category: Transcription regulator]]
[[Category: Transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:59:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:38:20 2008''

Revision as of 14:38, 27 July 2008

Image:2pex.png

Template:STRUCTURE 2pex

Structure of reduced C22S OhrR from Xanthamonas Campestris

Template:ABSTRACT PUBMED 18042459

About this Structure

2PEX is a Single protein structure of sequence from Xanthomonas campestris. Full crystallographic information is available from OCA.

Reference

Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:18042459

Page seeded by OCA on Sun Jul 27 17:38:20 2008

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