This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qmh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1qmh.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1qmh.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1qmh| PDB=1qmh | SCENE= }}
{{STRUCTURE_1qmh| PDB=1qmh | SCENE= }}
-
'''CRYSTAL STRUCTURE OF RNA 3'-TERMINAL PHOSPHATE CYCLASE, AN UBIQUITOUS ENZYME WITH UNUSUAL TOPOLOGY'''
+
===CRYSTAL STRUCTURE OF RNA 3'-TERMINAL PHOSPHATE CYCLASE, AN UBIQUITOUS ENZYME WITH UNUSUAL TOPOLOGY===
-
==Overview==
+
<!--
-
BACKGROUND: RNA cyclases are a family of RNA-modifying enzymes that are conserved in eucarya, bacteria and archaea. They catalyze the ATP-dependent conversion of the 3'-phosphate to the 2',3'-cyclic phosphodiester at the end of RNA, in a reaction involving formation of the covalent AMP-cyclase intermediate. These enzymes might be responsible for production of the cyclic phosphate RNA ends that are known to be required by many RNA ligases in both prokaryotes and eukaryotes. RESULTS: The high-resolution structure of the Escherichia coli RNA 3'-terminal phosphate cyclase was determined using multiwavelength anomalous diffraction. Two orthorhombic crystal forms of E. coli cyclase (space group P2(1)2(1)2(1) and P2(1)2(1)2) were used to solve and refine the structure to 2.1 A resolution (R factor 20.4%; R(free) 27.6%). Each molecule of RNA cyclase consists of two domains. The larger domain contains three repeats of a folding unit comprising two parallel alpha helices and a four-stranded beta sheet; this fold was previously identified in translation initiation factor 3 (IF3). The large domain is similar to one of the two domains of 5-enolpyruvylshikimate-3-phosphate synthase and UDP-N-acetylglucosamine enolpyruvyl transferase. The smaller domain uses a similar secondary structure element with different topology, observed in many other proteins such as thioredoxin. CONCLUSIONS: The fold of RNA cyclase consists of known elements connected in a new and unique manner. Although the active site of this enzyme could not be unambiguously assigned, it can be mapped to a region surrounding His309, an adenylate acceptor, in which a number of amino acids are highly conserved in the enzyme from different sources. The structure of E. coli cyclase will be useful for interpretation of structural and mechanistic features of this and other related enzymes.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10673421}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10673421 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10673421}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: 2'3'cyclic phosphate rna]]
[[Category: 2'3'cyclic phosphate rna]]
[[Category: Rna 3'-terminal phosphate cyclase]]
[[Category: Rna 3'-terminal phosphate cyclase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:27:09 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:56:31 2008''

Revision as of 15:56, 27 July 2008

Image:1qmh.png

Template:STRUCTURE 1qmh

CRYSTAL STRUCTURE OF RNA 3'-TERMINAL PHOSPHATE CYCLASE, AN UBIQUITOUS ENZYME WITH UNUSUAL TOPOLOGY

Template:ABSTRACT PUBMED 10673421

About this Structure

1QMH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology., Palm GJ, Billy E, Filipowicz W, Wlodawer A, Structure. 2000 Jan 15;8(1):13-23. PMID:10673421

Page seeded by OCA on Sun Jul 27 18:56:31 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qmh"
Personal tools