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1ztr

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[[Image:1ztr.gif|left|200px]]
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[[Image:1ztr.png|left|200px]]
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{{STRUCTURE_1ztr| PDB=1ztr | SCENE= }}
{{STRUCTURE_1ztr| PDB=1ztr | SCENE= }}
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'''Solution structure of Engrailed homeodomain L16A mutant'''
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===Solution structure of Engrailed homeodomain L16A mutant===
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==Overview==
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The most controversial area in protein folding concerns its earliest stages. Questions such as whether there are genuine folding intermediates, and whether the events at the earliest stages are just rearrangements of the denatured state or progress from populated transition states, remain unresolved. The problem is that there is a lack of experimental high-resolution structural information about early folding intermediates and denatured states under conditions that favour folding because competent states spontaneously fold rapidly. Here we have solved directly the solution structure of a true denatured state by nuclear magnetic resonance under conditions that would normally favour folding, and directly studied its equilibrium and kinetic behaviour. We engineered a mutant of Drosophila melanogaster Engrailed homeodomain that folds and unfolds reversibly just by changing ionic strength. At high ionic strength, the mutant L16A is an ultra-fast folding native protein, just like the wild-type protein; however, at physiological ionic strength it is denatured. The denatured state is a well-ordered folding intermediate, poised to fold by docking helices and breaking some non-native interactions. It unfolds relatively progressively with increasingly denaturing conditions, and so superficially resembles a denatured state with properties that vary with conditions. Such ill-defined unfolding is a common feature of early folding intermediate states and accounts for why there are so many controversies about intermediates versus compact denatured states in protein folding.
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(as it appears on PubMed at http://www.pubmed.gov), where 16222301 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16222301}}
==About this Structure==
==About this Structure==
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1ZTR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTR OCA].
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1ZTR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTR OCA].
==Reference==
==Reference==
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[[Category: Mutant]]
[[Category: Mutant]]
[[Category: Protein folding]]
[[Category: Protein folding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:03:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:01:48 2008''

Revision as of 16:01, 27 July 2008

Image:1ztr.png

Template:STRUCTURE 1ztr

Solution structure of Engrailed homeodomain L16A mutant

Template:ABSTRACT PUBMED 16222301

About this Structure

1ZTR is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Solution structure of a protein denatured state and folding intermediate., Religa TL, Markson JS, Mayor U, Freund SM, Fersht AR, Nature. 2005 Oct 13;437(7061):1053-6. PMID:16222301

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