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2q0r

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[[Image:2q0r.gif|left|200px]]
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[[Image:2q0r.png|left|200px]]
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{{STRUCTURE_2q0r| PDB=2q0r | SCENE= }}
{{STRUCTURE_2q0r| PDB=2q0r | SCENE= }}
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'''Structure of Pectenotoxin-2 Bound to Actin'''
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===Structure of Pectenotoxin-2 Bound to Actin===
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==Overview==
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(PTXs) are polyether macrolides found in certain dinoflagellates, sponges and shellfish, and have been associated with diarrhetic shellfish poisoning. In addition to their in vivo toxicity, some PTXs are potently cytotoxic in human cancer cell lines. Recent studies have demonstrated that disruption of the actin cytoskeleton may be a key function of these compounds, although no clarification of their mechanism of action at a molecular level was available. We have obtained an X-ray crystal structure of PTX-2 bound to actin, which, in combination with analyses of the effect of PTX-2 on purified actin filament dynamics, provides a molecular explanation for its effects on actin. PTX-2 formed a 1:1 complex with actin and engaged a novel site between subdomains 1 and 3. Based on models of the actin filament, PTX binding would disrupt key lateral contacts between the PTX-bound actin monomer and the lower lateral actin monomer within the filament, thereby capping the barbed-end. The location of this binding position within the interior of the filament indicates that it may not be accessible once polymerization has occurred, a hypothesis supported by our observation that PTX-2 caused filament capping without inducing filament severing. This mode of action is unique, as other actin filament destabilizing toxins appear to exclusively disrupt longitudinal monomer contacts, allowing many of them to sever filaments in addition to capping them. Examination of the PTX-binding site on actin provides a rationalization for the structure-activity relationships observed in vivo and in vitro, and may provide a basis for predicting toxicity of PTX analogues.
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(as it appears on PubMed at http://www.pubmed.gov), where 17599353 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17599353}}
==About this Structure==
==About this Structure==
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[[Category: Pectenotoxin]]
[[Category: Pectenotoxin]]
[[Category: Structural protein]]
[[Category: Structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:07:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:32:28 2008''

Revision as of 17:32, 27 July 2008

Image:2q0r.png

Template:STRUCTURE 2q0r

Structure of Pectenotoxin-2 Bound to Actin

Template:ABSTRACT PUBMED 17599353

About this Structure

2Q0R is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

A structural basis for regulation of actin polymerization by pectenotoxins., Allingham JS, Miles CO, Rayment I, J Mol Biol. 2007 Aug 24;371(4):959-70. Epub 2007 May 25. PMID:17599353

Page seeded by OCA on Sun Jul 27 20:32:28 2008

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