This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1nrb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nrb.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1nrb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1nrb| PDB=1nrb | SCENE= }}
{{STRUCTURE_1nrb| PDB=1nrb | SCENE= }}
-
'''SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING'''
+
===SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING===
-
==Overview==
+
<!--
-
We have determined the solution structure of an alpha-toxin, CsE-V, isolated from the venom of the New World scorpion Centruroides sculpturatus Ewing (CsE). This toxin causes spontaneous rhythmic contractions in muscle. Unlike other New World toxins from CsE, this protein exhibits amino acid insertions and deletions at locations similar to Old World toxins and may thus represent a transition protein between the New World and Old World scorpion alpha-toxins. Sequence-specific assignments were made using 600 MHz 1H two-dimensional NMR data. NOESY, PH-COSY and amide-exchange data were used to deduce constraints for molecular modeling calculations. Distance geometry and dynamical simulated annealing calculations were performed to generate a family of 70 structures free of constraint violations. With respect to this family of structures, the energy-minimized average structure had root-mean-square deviations of 0.74 and 1.32 A for backbone and all atoms, respectively (excluding the C-terminal dipeptide, which is disordered). As with other scorpion toxins, the secondary structure of CsE-V consists of an alpha-helix, a three-strand anti-parallel beta-sheet, four beta-turns, and a hydrophobic patch that includes tyrosine residues in herringbone configuration. Unlike the CsE-v3 and -v1 proteins from C. sculpturatus, all of the proline residues were found to be in the trans configuration. The alpha-helix is slightly longer in CsE-V. The overall structure is more similar to the Old World alpha-toxin AaH-II from Androctonus australis Hector (r.m.s.d 1.59 A for backbone atoms of matching residues) than to the New World alpha-toxin CsE-v3 (r.m.s.d. 1.91 A). These structural data on CsE-V add further to our knowledge of the conformational repertoire exhibited by these sodium channel-binding neurotoxins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_7739052}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 7739052 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_7739052}}
==About this Structure==
==About this Structure==
-
1NRB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NRB OCA].
+
1NRB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NRB OCA].
==Reference==
==Reference==
Line 25: Line 29:
[[Category: Krishna, N R.]]
[[Category: Krishna, N R.]]
[[Category: Neurotoxin]]
[[Category: Neurotoxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:53:27 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:50:18 2008''

Revision as of 17:50, 27 July 2008

Image:1nrb.png

Template:STRUCTURE 1nrb

SOLUTION STRUCTURE OF AN OLD WORLD-LIKE NEUROTOXIN FROM THE VENOM OF THE NEW WORLD SCORPION CENTRUROIDES SCULPTURATUS EWING

Template:ABSTRACT PUBMED 7739052

About this Structure

1NRB is a Single protein structure of sequence from Centruroides sculpturatus. Full experimental information is available from OCA.

Reference

Solution structure of an Old World-like neurotoxin from the venom of the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Watt DD, Krishna NR, J Mol Biol. 1995 Apr 28;248(2):449-58. PMID:7739052

Page seeded by OCA on Sun Jul 27 20:50:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nrb"
Personal tools