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1owl

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{{STRUCTURE_1owl| PDB=1owl | SCENE= }}
{{STRUCTURE_1owl| PDB=1owl | SCENE= }}
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'''Structure of apophotolyase from Anacystis nidulans'''
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===Structure of apophotolyase from Anacystis nidulans===
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==Overview==
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DNA photolyase is a unique flavoenzyme that repairs UV-induced DNA lesions using the energy of visible light. Anacystis nidulans photolyase contains a light-harvesting chromophore, 8-hydroxy-5-deazaflavin (8-HDF), and flavin adenine dinucleotide (FAD) which, in contrast to the 8-HDF chromophore, is indispensable for catalytic activity. This work reports the crystallization and structure at 1.8 A resolution of DNA photolyase devoid of its 8-HDF chromophore (apophotolyase). The overall three-dimensional structure is similar to that of the holoenzyme, indicating that the presence of 8-HDF is not essential for the correct folding of the enzyme. Structural changes include an additional phosphate group, a different conformation for Arg11 and slight rearrangements of Met47, Asp101 and Asp382, which replace part of the 8-HDF molecule in the chromophore-binding pocket. The apophotolyase can be efficiently reconstituted with synthetic 8-hydroxy-5-deazariboflavin, despite the orientation of Arg11 and the presence of the phosphate group in the 8-HDF pocket. Red light or X-rays reduced the FAD chromophore in apophotolyase crystals, as observed by single-crystal spectrophotometry. The structural effects of FAD reduction were determined by comparison of three data sets that were successively collected at 100 K, while the degree of reduction was monitored online by changes in the light absorption of the crystals. X-ray-induced conformational changes were confined to the active site of the protein. They include sub-angstrom movements of the O(2) and N(5) atoms of the flavin group as well as the O(delta) atoms of the surrounding amino acids Asp380 and Asn386.
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(as it appears on PubMed at http://www.pubmed.gov), where 15213381 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15213381}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction., Kort R, Komori H, Adachi S, Miki K, Eker A, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1205-13. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213381 15213381]
DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction., Kort R, Komori H, Adachi S, Miki K, Eker A, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1205-13. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213381 15213381]
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Crystallography &amp; NMR system: A new software suite for macromolecular structure determination., Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges M, Pannu NS, Read RJ, Rice LM, Simonson T, Warren GL, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9757107 9757107]
[[Category: Deoxyribodipyrimidine photo-lyase]]
[[Category: Deoxyribodipyrimidine photo-lyase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Flavin enzyme]]
[[Category: Flavin enzyme]]
[[Category: Photoreactivating enzyme]]
[[Category: Photoreactivating enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:22:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:00:07 2008''

Revision as of 18:00, 27 July 2008

Image:1owl.png

Template:STRUCTURE 1owl

Structure of apophotolyase from Anacystis nidulans

Template:ABSTRACT PUBMED 15213381

About this Structure

1OWL is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.

Reference

DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction., Kort R, Komori H, Adachi S, Miki K, Eker A, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1205-13. Epub 2004, Jun 22. PMID:15213381

Crystallography & NMR system: A new software suite for macromolecular structure determination., Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges M, Pannu NS, Read RJ, Rice LM, Simonson T, Warren GL, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21. PMID:9757107

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