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2jf6

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{{STRUCTURE_2jf6| PDB=2jf6 | SCENE= }}
{{STRUCTURE_2jf6| PDB=2jf6 | SCENE= }}
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'''STRUCTURE OF INACTIVE MUTANT OF STRICTOSIDINE GLUCOSIDASE IN COMPLEX WITH STRICTOSIDINE'''
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===STRUCTURE OF INACTIVE MUTANT OF STRICTOSIDINE GLUCOSIDASE IN COMPLEX WITH STRICTOSIDINE===
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==Overview==
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Strictosidine beta-D-glucosidase (SG) follows strictosidine synthase (STR1) in the production of the reactive intermediate required for the formation of the large family of monoterpenoid indole alkaloids in plants. This family is composed of approximately 2000 structurally diverse compounds. SG plays an important role in the plant cell by activating the glucoside strictosidine and allowing it to enter the multiple indole alkaloid pathways. Here, we report detailed three-dimensional information describing both native SG and the complex of its inactive mutant Glu207Gln with the substrate strictosidine, thus providing a structural characterization of substrate binding and identifying the amino acids that occupy the active site surface of the enzyme. Structural analysis and site-directed mutagenesis experiments demonstrate the essential role of Glu-207, Glu-416, His-161, and Trp-388 in catalysis. Comparison of the catalytic pocket of SG with that of other plant glucosidases demonstrates the structural importance of Trp-388. Compared with all other glucosidases of plant, bacterial, and archaeal origin, SG's residue Trp-388 is present in a unique structural conformation that is specific to the SG enzyme. In addition to STR1 and vinorine synthase, SG represents the third structural example of enzymes participating in the biosynthetic pathway of the Rauvolfia alkaloid ajmaline. The data presented here will contribute to deciphering the structure and reaction mechanism of other higher plant glucosidases.
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(as it appears on PubMed at http://www.pubmed.gov), where 17890378 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17890378}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Strictosidine]]
[[Category: Strictosidine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:49:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:13:52 2008''

Revision as of 18:13, 27 July 2008

Image:2jf6.png

Template:STRUCTURE 2jf6

STRUCTURE OF INACTIVE MUTANT OF STRICTOSIDINE GLUCOSIDASE IN COMPLEX WITH STRICTOSIDINE

Template:ABSTRACT PUBMED 17890378

About this Structure

2JF6 is a Single protein structure of sequence from Rauvolfia serpentina. Full crystallographic information is available from OCA.

Reference

Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family., Barleben L, Panjikar S, Ruppert M, Koepke J, Stockigt J, Plant Cell. 2007 Sep;19(9):2886-97. Epub 2007 Sep 21. PMID:17890378

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