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2q6a

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{{STRUCTURE_2q6a| PDB=2q6a | SCENE= }}
{{STRUCTURE_2q6a| PDB=2q6a | SCENE= }}
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'''Crystal Structure of Nak channel D66E mutant'''
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===Crystal Structure of Nak channel D66E mutant===
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==Overview==
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Apparent blockage of monovalent cation currents by the permeating blocker Ca(2+) is a physiologically essential phenomenon relevant to cyclic nucleotide-gated (CNG) channels. The recently determined crystal structure of a bacterial homolog of CNG channel pores, the NaK channel, revealed a Ca(2+) binding site at the extracellular entrance to the selectivity filter. This site is not formed by the side-chain carboxylate groups from the conserved acidic residue, Asp-66 in NaK, conventionally thought to directly chelate Ca(2+) in CNG channels, but rather by the backbone carbonyl groups of residue Gly-67. Here we present a detailed structural analysis of the NaK channel with a focus on Ca(2+) permeability and blockage. Our results confirm that the Asp-66 residue, although not involved in direct chelation of Ca(2+), plays an essential role in external Ca(2+) binding. Furthermore, we give evidence for the presence of a second Ca(2+) binding site within the NaK selectivity filter where monovalent cations also bind, providing a structural basis for Ca(2+) permeation through the NaK pore. Compared with other Ca(2+)-binding proteins, both sites in NaK present a novel mode of Ca(2+) chelation, using only backbone carbonyl oxygen atoms from residues in the selectivity filter. The external site is under indirect control by an acidic residue (Asp-66), making it Ca(2+)-specific. These findings give us a glimpse of the possible underlying mechanisms allowing Ca(2+) to act both as a permeating ion and blocker of CNG channels and raise the possibility of a similar chemistry governing Ca(2+) chelation in Ca(2+) channels.
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(as it appears on PubMed at http://www.pubmed.gov), where 17878296 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17878296}}
==About this Structure==
==About this Structure==
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[[Category: Metal transport]]
[[Category: Metal transport]]
[[Category: Tetramer]]
[[Category: Tetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:26:28 2008''

Revision as of 18:26, 27 July 2008

Image:2q6a.png

Template:STRUCTURE 2q6a

Crystal Structure of Nak channel D66E mutant

Template:ABSTRACT PUBMED 17878296

About this Structure

2Q6A is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.

Reference

Structural insight into Ca2+ specificity in tetrameric cation channels., Alam A, Shi N, Jiang Y, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15334-9. Epub 2007 Sep 18. PMID:17878296

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