2er8

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[[Image:2er8.gif|left|200px]]
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{{STRUCTURE_2er8| PDB=2er8 | SCENE= }}
{{STRUCTURE_2er8| PDB=2er8 | SCENE= }}
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'''Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex'''
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===Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex===
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==Overview==
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Gal4 is the prototypical Zn2Cys6 binuclear cluster transcriptional regulator that binds as a homodimer to DNA containing inverted CGG half-sites. Leu3, a member of this protein family, binds to everted (opposite polarity to inverted) CGG half-sites, and an H50C mutation within the Leu3 Zn2Cys6 binuclear motif abolishes its transcriptional repression function without impairing DNA binding. We report the X-ray crystal structures of DNA complexes with Leu3 and Leu3(H50C) and solution DNA binding studies of selected Leu3 mutant proteins. These studies reveal the molecular details of everted CGG half-site recognition, and suggest a role for the H50C mutation in transcriptional repression. Comparison with the Gal4-DNA complex shows an unexpected conservation in the DNA recognition mode of inverted and everted CGG half-sites, and points to a critical function of a linker region between the Zn2Cys6 binuclear cluster and dimerization regions in DNA binding specificity. Broader implications of these findings are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 16615914 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16615914}}
==About this Structure==
==About this Structure==
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[[Category: Fitzgerald, M X.]]
[[Category: Fitzgerald, M X.]]
[[Category: Marmorstein, R.]]
[[Category: Marmorstein, R.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:01:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:27:23 2008''

Revision as of 18:27, 27 July 2008

Image:2er8.png

Template:STRUCTURE 2er8

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex

Template:ABSTRACT PUBMED 16615914

About this Structure

2ER8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein., Fitzgerald MX, Rojas JR, Kim JM, Kohlhaw GB, Marmorstein R, Structure. 2006 Apr;14(4):725-35. PMID:16615914

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