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1sjs

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[[Image:1sjs.gif|left|200px]]
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[[Image:1sjs.png|left|200px]]
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{{STRUCTURE_1sjs| PDB=1sjs | SCENE= }}
{{STRUCTURE_1sjs| PDB=1sjs | SCENE= }}
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'''ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING'''
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===ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING===
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==Overview==
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To clarify further the mechanism of regulation by phosphorylation of isocitrate dehydrogenase, cocrystallization of isocitrate dehydrogenase and isocitrate dehydrogenase kinase/phosphatase in the presence of an ATP analog was attempted. Although cocrystallization was unsuccessful, a new crystal form of isocitrate dehydrogenase was obtained which provides insight into the phosphorylation mechanism. The new, orthorhombic crystal form of isocitrate dehydrogenase is related to the previously reported tetragonal form largely by an approximately 16 degrees shift of a large domain relative to the small domain and clasp region within each subunit of the dimeric enzyme. The NADP+ cofactor binding surface is significantly disrupted by the shift to the open conformation. The solvent-accessible surface area and surface-enclosed volume increase by 2% relative to the dimeric tetragonal form. Most of the increase results from expansion of the active site cleft such that the distance across its opening increases from approximately 5 to 13 A, significantly increasing accessibility to Ser-113. The conformation of isocitrate dehydrogenase in the orthorhombic crystal form more closely resembles that of the crystal structure of the homologous enzyme 3-isopropylmalate dehydrogenase than does the tetragonal isocitrate dehydrogenase conformation. Since the crystal lattice forces are fairly weak, it appears that isocitrate dehydrogenase is a flexible molecule that can easily undergo domain shifts and possibly other induced fit conformational changes, to accommodate binding to isocitrate dehydrogenase kinase/phosphatase.
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(as it appears on PubMed at http://www.pubmed.gov), where 9374867 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9374867}}
==About this Structure==
==About this Structure==
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[[Category: I oxidoreductase]]
[[Category: I oxidoreductase]]
[[Category: Phosphorylation]]
[[Category: Phosphorylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:47:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:46:01 2008''

Revision as of 19:46, 27 July 2008

Image:1sjs.png

Template:STRUCTURE 1sjs

ACCESS TO PHOSPHORYLATION IN ISOCITRATE DEHYDROGENASE MAY OCCUR BY DOMAIN SHIFTING

Template:ABSTRACT PUBMED 9374867

About this Structure

1SJS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Access to phosphorylation in isocitrate dehydrogenase may occur by domain shifting., Finer-Moore J, Tsutakawa SE, Cherbavaz DR, LaPorte DC, Koshland DE Jr, Stroud RM, Biochemistry. 1997 Nov 11;36(45):13890-6. PMID:9374867

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