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1ss7

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{{STRUCTURE_1ss7| PDB=1ss7 | SCENE= }}
{{STRUCTURE_1ss7| PDB=1ss7 | SCENE= }}
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'''Compensating bends in a 16 base-pair DNA oligomer containing a T3A3 segment'''
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===Compensating bends in a 16 base-pair DNA oligomer containing a T3A3 segment===
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==Overview==
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In-phase ligated DNA containing T(n)A(n) segments fail to exhibit the retarded polyacrylamide gel electrophoresis (PAGE) migration observed for in-phase ligated A(n)T(n) segments, a behavior thought to be correlated with macroscopic DNA curvature. The lack of macroscopic curvature in ligated T(n)A(n) segments is thought to be due to cancellation of bending in regions flanking the TpA steps. To address this issue, solution-state NMR, including residual dipolar coupling (RDC) restraints, was used to determine a high-resolution structure of [d(CGAGGTTTAAACCTCG)2], a DNA oligomer containing a T3A3 tract. The overall magnitude and direction of bending, including the regions flanking the central TpA step, was measured using a radius of curvature, Rc, analysis. The Rc for the overall molecule indicated a small magnitude of global bending (Rc = 138 +/- 23 nm) towards the major groove, whereas the Rc for the two halves (72 +/- 33 nm and 69 +/- 14 nm) indicated greater localized bending into the minor groove. The direction of bending in the regions flanking the TpA step is in partial opposition (109 degrees), contributing to cancellation of bending. The cancellation of bending did not correlate with a pattern of roll values at the TpA step, or at the 5' and 3' junctions, of the T3A3 segment, suggesting a simple junction/roll model is insufficient to predict cancellation of DNA bending in all T(n)A(n) junction sequence contexts. Importantly, Rc analysis of structures refined without RDC restraints lacked the precision and accuracy needed to reliably measure bending.
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(as it appears on PubMed at http://www.pubmed.gov), where 15526287 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15526287}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SS7 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SS7 OCA].
==Reference==
==Reference==
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Residual dipolar coupling]]
[[Category: Residual dipolar coupling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:04:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:46:58 2008''

Revision as of 19:47, 27 July 2008

Image:1ss7.png

Template:STRUCTURE 1ss7

Compensating bends in a 16 base-pair DNA oligomer containing a T3A3 segment

Template:ABSTRACT PUBMED 15526287

About this Structure

Full experimental information is available from OCA.

Reference

Compensating bends in a 16-base-pair DNA oligomer containing a T(3)A(3) segment: A NMR study of global DNA curvature., McAteer K, Aceves-Gaona A, Michalczyk R, Buchko GW, Isern NG, Silks LA, Miller JH, Kennedy MA, Biopolymers. 2004 Dec 15;75(6):497-511. PMID:15526287

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