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1po5

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[[Image:1po5.gif|left|200px]]
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{{STRUCTURE_1po5| PDB=1po5 | SCENE= }}
{{STRUCTURE_1po5| PDB=1po5 | SCENE= }}
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'''Structure of mammalian cytochrome P450 2B4'''
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===Structure of mammalian cytochrome P450 2B4===
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==Overview==
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The xenobiotic metabolizing cytochromes P450 (P450s) are among the most versatile biological catalysts known, but knowledge of the structural basis for their broad substrate specificity has been limited. P450 2B4 has been frequently used as an experimental model for biochemical and biophysical studies of these membrane proteins. A 1.6-A crystal structure of P450 2B4 reveals a large open cleft that extends from the protein surface directly to the heme iron between the alpha-helical and beta-sheet domains without perturbing the overall P450 fold. This cleft is primarily formed by helices B' to C and F to G. The conformation of these regions is dramatically different from that of the other structurally defined mammalian P450, 2C5/3LVdH, in which the F to G and B' to C regions encapsulate one side of the active site to produce a closed form of the enzyme. The open conformation of 2B4 is trapped by reversible formation of a homodimer in which the residues between helices F and G of one molecule partially fill the open cleft of a symmetry-related molecule, and an intermolecular coordinate bond occurs between H226 and the heme iron. This dimer is observed both in solution and in the crystal. Differences between the structures of 2C5 and 2B4 suggest that defined regions of xenobiotic metabolizing P450s may adopt a substantial range of energetically accessible conformations without perturbing the overall fold. This conformational flexibility is likely to facilitate substrate access, metabolic versatility, and product egress.
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{{ABSTRACT_PUBMED_14563924}}
==About this Structure==
==About this Structure==
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[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:17:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:14:07 2008''

Revision as of 20:14, 27 July 2008

Image:1po5.png

Template:STRUCTURE 1po5

Structure of mammalian cytochrome P450 2B4

Template:ABSTRACT PUBMED 14563924

About this Structure

1PO5 is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution., Scott EE, He YA, Wester MR, White MA, Chin CC, Halpert JR, Johnson EF, Stout CD, Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13196-201. Epub 2003 Oct 16. PMID:14563924

Page seeded by OCA on Sun Jul 27 23:14:07 2008

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