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| | {{STRUCTURE_1v91| PDB=1v91 | SCENE= }} | | {{STRUCTURE_1v91| PDB=1v91 | SCENE= }} |
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| - | '''Solution structure of insectidal toxin delta-paluIT2-NH2'''
| + | ===Solution structure of insectidal toxin delta-paluIT2-NH2=== |
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| - | ==Overview==
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| - | Delta-paluIT1 and delta-paluIT2 are toxins purified from the venom of the spider Paracoelotes luctuosus. Similar in sequence to mu-agatoxins from Agelenopsis aperta, their pharmacological target is the voltage-gated insect sodium channel, of which they alter the inactivation properties in a way similar to alpha-scorpion toxins, but they bind on site 4 in a way similar to beta-scorpion toxins. We determined the solution structure of the two toxins by use of two-dimensional nuclear magnetic resonance (NMR) techniques followed by distance geometry and molecular dynamics. The structures of delta-paluIT1 and delta-paluIT2 belong to the inhibitory cystine knot structural family, i.e. a compact disulfide-bonded core from which four loops emerge. Delta-paluIT1 and delta-paluIT2 contain respectively two- and three-stranded anti-parallel beta-sheets as unique secondary structure. We compare the structure and the electrostatic anisotropy of those peptides to other sodium and calcium channel toxins, analyze the topological juxtaposition of key functional residues, and conclude that the recognition of insect voltage-gated sodium channels by these toxins involves the beta-sheet, in addition to loops I and IV. Besides the position of culprit residues on the molecular surface, difference in dipolar moment orientation is another determinant of receptor binding and biological activity differences. We also demonstrate by electrophysiological experiments on the cloned insect voltage-gated sodium channel, para, heterologuously co-expressed with the tipE subunit in Xenopus laevis oocytes, that delta-paluIT1 and delta-paluIT2 procure an increase of Na+ current. delta-PaluIT1-OH seems to have less effect when the same concentrations are used.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15726637}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15726637 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15726637}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1V91 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoelotes_luctuosus Paracoelotes luctuosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V91 OCA]. | + | 1V91 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoelotes_luctuosus Paracoelotes luctuosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V91 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Neurotoxin]] | | [[Category: Neurotoxin]] |
| | [[Category: Sodium channel inhibitor]] | | [[Category: Sodium channel inhibitor]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:14:35 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:40:28 2008'' |
Revision as of 20:40, 27 July 2008
Template:STRUCTURE 1v91
Solution structure of insectidal toxin delta-paluIT2-NH2
Template:ABSTRACT PUBMED 15726637
About this Structure
1V91 is a Single protein structure of sequence from Paracoelotes luctuosus. Full experimental information is available from OCA.
Reference
Solution structure of two insect-specific spider toxins and their pharmacological interaction with the insect voltage-gated Na+ channel., Ferrat G, Bosmans F, Tytgat J, Pimentel C, Chagot B, Gilles N, Nakajima T, Darbon H, Corzo G, Proteins. 2005 May 1;59(2):368-79. PMID:15726637
Page seeded by OCA on Sun Jul 27 23:40:28 2008
