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1zym

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{{STRUCTURE_1zym| PDB=1zym | SCENE= }}
{{STRUCTURE_1zym| PDB=1zym | SCENE= }}
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'''AMINO TERMINAL DOMAIN OF ENZYME I FROM ESCHERICHIA COLI'''
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===AMINO TERMINAL DOMAIN OF ENZYME I FROM ESCHERICHIA COLI===
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==Overview==
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BACKGROUND: The bacterial phosphoenolpyruvate (PEP): sugar phosphotransferase system (PTS) transports exogenous hexose sugars through the membrane and tightly couples transport with phosphoryl transfer from PEP to the sugar via several phosphoprotein intermediates. The phosphate group is first transferred to enzyme I, second to the histidine-containing phosphocarrier protein HPr, and then to one of a number of sugar-specific enzymes II. The structures of several HPrs and enzymes IIA are known. Here we report the structure of the N-terminal half of enzyme I from Escherichia coli (EIN). RESULTS: The crystal structure of EIN (MW approximately 30 kDa) has been determined and refined at 2.5 A resolution. It has two distinct structural subdomains; one contains four alpha helices arranged as two hairpins in a claw-like conformation. The other consists of a beta sandwich containing a three-stranded antiparallel beta sheet and a four-stranded parallel beta sheet, together with three short alpha helices. Plausible models of complexes between EIN and HPr can be made without assuming major structural changes in either protein. CONCLUSIONS: The alpha/beta subdomain of EIN is topologically similar to the phosphohistidine domain of the enzyme pyruvate phosphate dikinase, which is phosphorylated by PEP on a histidyl residue but does not interact with HPr. It is therefore likely that features of this subdomain are important in the autophosphorylation of enzyme I. The helical subdomain of EIN is not found in pyruvate phosphate dikinase; this subdomain is therefore more likely to be involved in phosphoryl transfer to HPr.
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(as it appears on PubMed at http://www.pubmed.gov), where 8805571 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8805571}}
==About this Structure==
==About this Structure==
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[[Category: Liao, D I.]]
[[Category: Liao, D I.]]
[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:14:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:46:04 2008''

Revision as of 20:46, 27 July 2008

Image:1zym.png

Template:STRUCTURE 1zym

AMINO TERMINAL DOMAIN OF ENZYME I FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 8805571

About this Structure

1ZYM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The first step in sugar transport: crystal structure of the amino terminal domain of enzyme I of the E. coli PEP: sugar phosphotransferase system and a model of the phosphotransfer complex with HPr., Liao DI, Silverton E, Seok YJ, Lee BR, Peterkofsky A, Davies DR, Structure. 1996 Jul 15;4(7):861-72. PMID:8805571

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