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1t9h

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{{STRUCTURE_1t9h| PDB=1t9h | SCENE= }}
{{STRUCTURE_1t9h| PDB=1t9h | SCENE= }}
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'''The crystal structure of YloQ, a circularly permuted GTPase.'''
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===The crystal structure of YloQ, a circularly permuted GTPase.===
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==Overview==
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yloQ is one of 11 essential genes in Bacillus subtilis with unknown roles in the physiology of the cell. It encodes a polypeptide of 298 residues with motifs characteristic of GTPases. As a contribution to elucidating its indispensable cellular function, we have solved the crystal structure of YloQ to 1.6 A spacing, revealing a three-domain organisation. At the heart of the molecule is the putative GTPase domain, which exhibits a classical alpha/beta nucleotide-binding fold with a topology very similar to that of Ras and Era. However, as anticipated from the order in which the conserved G protein motifs appear in the sequence, the GTPase domain fold in YloQ is circularly permuted with respect to the classical GTPases. The nucleotide-binding pocket in YloQ is unoccupied, and analysis of the phosphate-binding (P) loop indicates that conformational changes in this region would be needed to accommodate GTP. The GTPase domain is flanked at its N terminus by a beta-barrel domain with an oligonucleotide/oligosaccharide-binding (OB) fold, and at its C terminus by an alpha-helical domain containing a coordinated zinc ion. This combination of protein modules is unique to YloQ and its orthologues. Sequence comparisons reveal a clustering of conserved basic and aromatic residues on one face of the OB domain, perhaps pointing to a role for YloQ in nucleic acid binding. The zinc ion in the alpha-helical domain is coordinated by three cysteine residues and a histidine residue in a novel ligand organisation. The juxtaposition of the switch I and switch II regions of the G domain and the OB and zinc-binding domains suggests that chemical events at the GTPase active site may be transduced into relative movements of these domains. The pattern of conserved residues and electrostatic surface potential calculations suggest that the OB and/or Zn-binding domains participate in nucleic acid binding consistent with a possible role for YloQ at some stage during mRNA translation.
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(as it appears on PubMed at http://www.pubmed.gov), where 15223319 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15223319}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability., Levdikov VM, Blagova EV, Brannigan JA, Cladiere L, Antson AA, Isupov MN, Seror SJ, Wilkinson AJ, J Mol Biol. 2004 Jul 16;340(4):767-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15223319 15223319]
The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability., Levdikov VM, Blagova EV, Brannigan JA, Cladiere L, Antson AA, Isupov MN, Seror SJ, Wilkinson AJ, J Mol Biol. 2004 Jul 16;340(4):767-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15223319 15223319]
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Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability., Cladiere L, Blagova E, Levdikov VM, Brannigan JA, Seror SJ, Wilkinson AJ, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):329-30. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747714 14747714]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Central gtp binding domain]]
[[Category: Central gtp binding domain]]
[[Category: N-terminal beta-barrel domain with oligonucleotide binding fold]]
[[Category: N-terminal beta-barrel domain with oligonucleotide binding fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:42:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:51:52 2008''

Revision as of 20:51, 27 July 2008

Image:1t9h.png

Template:STRUCTURE 1t9h

The crystal structure of YloQ, a circularly permuted GTPase.

Template:ABSTRACT PUBMED 15223319

About this Structure

1T9H is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability., Levdikov VM, Blagova EV, Brannigan JA, Cladiere L, Antson AA, Isupov MN, Seror SJ, Wilkinson AJ, J Mol Biol. 2004 Jul 16;340(4):767-82. PMID:15223319

Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability., Cladiere L, Blagova E, Levdikov VM, Brannigan JA, Seror SJ, Wilkinson AJ, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):329-30. Epub 2004, Jan 23. PMID:14747714

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