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1siu

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[[Image:1siu.jpg|left|200px]]
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{{STRUCTURE_1siu| PDB=1siu | SCENE= }}
{{STRUCTURE_1siu| PDB=1siu | SCENE= }}
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'''KUMAMOLISIN-AS E78H MUTANT'''
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===KUMAMOLISIN-AS E78H MUTANT===
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==Overview==
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Kumamolisin-As (previously called ScpA) is the first known example of a collagenase from the sedolisin family (MEROPS S53). This enzyme is active at low pH and in elevated temperatures. In this study that used x-ray crystallographic and biochemical methods, we investigated the structural basis of the preference of this enzyme for collagen and the importance of a glutamate residue in the unique catalytic triad (Ser(278)-Glu(78)-Asp(82)) for enzymatic activity. Crystal structures of the uninhibited enzyme and its complex with a covalently bound inhibitor, N-acetyl-isoleucyl-prolyl-phenylalaninal, showed the occurrence of a narrow S2 pocket and a groove that encompasses the active site and is rich in negative charges. Limited endoproteolysis studies of bovine type-I collagen as well as kinetic studies using peptide libraries randomized at P1 and P1', showed very strong preference for arginine at the P1 position, which correlated very well with the presence of a negatively charged residue in the S1 pocket of the enzyme. All of these features, together with those predicted through comparisons with fiddler crab collagenase, a serine peptidase, rationalize the enzyme's preference for collagen. A comparison of the Arrhenius plots of the activities of kumamolisin-As with either collagen or peptides as substrates suggests that collagen should be relaxed before proteolysis can occur. The E78H mutant, in which the catalytic triad was engineered to resemble that of subtilisin, showed only 0.01% activity of the wild-type enzyme, and its structure revealed that Ser(278), His(78), and Asp(82) do not interact with each other; thus, the canonical catalytic triad is disrupted.
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(as it appears on PubMed at http://www.pubmed.gov), where 15014068 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15014068}}
==About this Structure==
==About this Structure==
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[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
[[Category: E78h]]
[[Category: E78h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:11:29 2008''

Revision as of 21:11, 27 July 2008

Image:1siu.png

Template:STRUCTURE 1siu

KUMAMOLISIN-AS E78H MUTANT

Template:ABSTRACT PUBMED 15014068

About this Structure

1SIU is a Single protein structure of sequence from Alicyclobacillus sendaiensis. Full crystallographic information is available from OCA.

Reference

Crystallographic and biochemical investigations of kumamolisin-As, a serine-carboxyl peptidase with collagenase activity., Wlodawer A, Li M, Gustchina A, Tsuruoka N, Ashida M, Minakata H, Oyama H, Oda K, Nishino T, Nakayama T, J Biol Chem. 2004 May 14;279(20):21500-10. Epub 2004 Mar 10. PMID:15014068

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