2uwb

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[[Image:2uwb.gif|left|200px]]
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{{Seed}}
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[[Image:2uwb.png|left|200px]]
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{{STRUCTURE_2uwb| PDB=2uwb | SCENE= }}
{{STRUCTURE_2uwb| PDB=2uwb | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING MUTANT XYLOGLUCANASE ISOFORM NXG1-DELTA-YNIIG'''
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===CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING MUTANT XYLOGLUCANASE ISOFORM NXG1-DELTA-YNIIG===
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==Overview==
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High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen (Populus tremula x Populus tremuloides). Production of the loop deletion variant Tm-NXG1-DeltaYNIIG yielded an enzyme that was structurally similar to Ptt-XET16-34 and had a greatly increased transglycosylation:hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.
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(as it appears on PubMed at http://www.pubmed.gov), where 17557806 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17557806}}
==About this Structure==
==About this Structure==
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[[Category: Tropaeolum majus xyloglucanase]]
[[Category: Tropaeolum majus xyloglucanase]]
[[Category: Xyloglucan-endo-transferase]]
[[Category: Xyloglucan-endo-transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:39:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:41:46 2008''

Revision as of 21:41, 27 July 2008

Image:2uwb.png

Template:STRUCTURE 2uwb

CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING MUTANT XYLOGLUCANASE ISOFORM NXG1-DELTA-YNIIG

Template:ABSTRACT PUBMED 17557806

About this Structure

2UWB is a Single protein structure of sequence from Tropaeolum majus. Full crystallographic information is available from OCA.

Reference

Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism., Baumann MJ, Eklof JM, Michel G, Kallas AM, Teeri TT, Czjzek M, Brumer H 3rd, Plant Cell. 2007 Jun;19(6):1947-63. Epub 2007 Jun 8. PMID:17557806

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