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2hap

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{{STRUCTURE_2hap| PDB=2hap | SCENE= }}
{{STRUCTURE_2hap| PDB=2hap | SCENE= }}
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'''STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION'''
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===STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION===
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==Overview==
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HAP1 is a yeast transcriptional activator that binds with equal affinity to the dissimilar upstream activation sequences UAS1 and UAS(CYC7), but activates transcription differentially when bound to each site. HAP1-18 harbors an amino acid change in the DNA binding domain. While binding UAS1 poorly, HAP1-18 binds UAS(CYC7) with wild-type properties and activates transcription at elevated levels relative to HAP1. We have determined the structure of HAP1-18-UAS(CYC7) and have compared it to HAP1-UAS(CYC7). Unexpectedly, the single amino acid substitution in HAP1-18 nucleates a significantly altered hydrogen bond interface between the protein and DNA resulting in DNA conformational changes and an ordering of one N-terminal arm of the protein dimer along the DNA minor groove. These observations, together with a large subset of transcriptionally defective mutations in the HAP1 DNA-binding domain that map to the HAP1-DNA interface, suggest that protein-DNA interactions may have direct allosteric effects on transcriptional activation.
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{{ABSTRACT_PUBMED_9886287}}
==About this Structure==
==About this Structure==
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[[Category: Hyperactive mutant]]
[[Category: Hyperactive mutant]]
[[Category: Transcriptional activation]]
[[Category: Transcriptional activation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:03:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:47:07 2008''

Revision as of 21:47, 27 July 2008

Image:2hap.png

Template:STRUCTURE 2hap

STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION

Template:ABSTRACT PUBMED 9886287

About this Structure

2HAP is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of HAP1-18-DNA implicates direct allosteric effect of protein-DNA interactions on transcriptional activation., King DA, Zhang L, Guarente L, Marmorstein R, Nat Struct Biol. 1999 Jan;6(1):22-7. PMID:9886287

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