1v7p

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[[Image:1v7p.gif|left|200px]]
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[[Image:1v7p.png|left|200px]]
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{{STRUCTURE_1v7p| PDB=1v7p | SCENE= }}
{{STRUCTURE_1v7p| PDB=1v7p | SCENE= }}
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'''Structure of EMS16-alpha2-I domain complex'''
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===Structure of EMS16-alpha2-I domain complex===
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==Overview==
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Snake venoms contain a number of heterodimeric C-type lectin-like proteins (CLPs) that interact specifically with components of the haemostatic system. EMS16 from the venom of Echis multisquamatus binds to the collagen receptor, integrin alpha2beta1, also known as glycoprotein (GP) Ia/IIa, and specifically inhibits collagen binding. Here we report the crystal structure of EMS16 in complex with recombinant integrin alpha2-I domain that plays a central role in collagen binding. The structure of the complex at 1.9 Angstrom resolution reveals that the collagen-binding site of the alpha2-I domain is covered completely by the bound EMS16. This blockage by EMS16 appears to spatially inhibit collagen binding to the alpha2-I domain. The bound alpha2-I domain adopts a closed conformation, which is seen in the absence of ligand, suggesting that EMS16 stabilizes a closed conformation corresponding to the less active structure of the alpha2-I domain. EMS16 does not directly bind to the manganese ion and residues of the metal ion-dependent adhesion site (MIDAS) of the alpha2-I domain, suggesting that EMS16 may have the potential to bind specifically to the alpha2-I domain in a metal ion-independent fashion.
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(as it appears on PubMed at http://www.pubmed.gov), where 15276841 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15276841}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of EMS16 in complex with the integrin alpha2-I domain., Horii K, Okuda D, Morita T, Mizuno H, J Mol Biol. 2004 Aug 6;341(2):519-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15276841 15276841]
Crystal structure of EMS16 in complex with the integrin alpha2-I domain., Horii K, Okuda D, Morita T, Mizuno H, J Mol Biol. 2004 Aug 6;341(2):519-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15276841 15276841]
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Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus., Horii K, Okuda D, Morita T, Mizuno H, Biochemistry. 2003 Nov 4;42(43):12497-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14580195 14580195]
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Crystal structure of the I domain from integrin alpha2beta1., Emsley J, King SL, Bergelson JM, Liddington RC, J Biol Chem. 1997 Nov 7;272(45):28512-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9353312 9353312]
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Structural basis of collagen recognition by integrin alpha2beta1., Emsley J, Knight CG, Farndale RW, Barnes MJ, Liddington RC, Cell. 2000 Mar 31;101(1):47-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10778855 10778855]
[[Category: Echis multisquamatus]]
[[Category: Echis multisquamatus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Integrin]]
[[Category: Integrin]]
[[Category: Snake venom]]
[[Category: Snake venom]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:11:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:49:34 2008''

Revision as of 21:49, 27 July 2008

Image:1v7p.png

Template:STRUCTURE 1v7p

Structure of EMS16-alpha2-I domain complex

Template:ABSTRACT PUBMED 15276841

About this Structure

1V7P is a Protein complex structure of sequences from Echis multisquamatus and Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of EMS16 in complex with the integrin alpha2-I domain., Horii K, Okuda D, Morita T, Mizuno H, J Mol Biol. 2004 Aug 6;341(2):519-27. PMID:15276841

Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus., Horii K, Okuda D, Morita T, Mizuno H, Biochemistry. 2003 Nov 4;42(43):12497-502. PMID:14580195

Crystal structure of the I domain from integrin alpha2beta1., Emsley J, King SL, Bergelson JM, Liddington RC, J Biol Chem. 1997 Nov 7;272(45):28512-7. PMID:9353312

Structural basis of collagen recognition by integrin alpha2beta1., Emsley J, Knight CG, Farndale RW, Barnes MJ, Liddington RC, Cell. 2000 Mar 31;101(1):47-56. PMID:10778855

Page seeded by OCA on Mon Jul 28 00:49:34 2008

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