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2grk

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{{STRUCTURE_2grk| PDB=2grk | SCENE= }}
{{STRUCTURE_2grk| PDB=2grk | SCENE= }}
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'''Crystal structure of ectromelia virus EVM1 chemokine binding protein'''
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===Crystal structure of ectromelia virus EVM1 chemokine binding protein===
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==Overview==
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The EVM1 protein encoded by Ectromelia virus is a member of a highly conserved family of poxvirus chemokine binding proteins that interfere with host immune surveillance processes. EVM1 is abundantly expressed early during mousepox infection and is able to selectively bind CC chemokines and inhibit their interactions with host receptors. Here, we characterize the interaction between EVM1 and the human and murine chemokines CCL3 (MIP-1alpha), CCL2 (MCP-1), and CCL5 (RANTES). Each of these CC chemokines binds EVM1 with 1:1 stoichiometry and equilibrium dissociation constants ranging from 29 pM to 20 nM. The interactions are characterized by rapid-association kinetics between acidic EVM1 and generally basic chemokines with half-lives enduring up to 30 min. The 2.6-A crystal structure of EVM1 reveals a globular beta sandwich with a large, sequence-conserved surface patch encircled by acidic residues on one face of the protein. To determine whether this conserved cluster of residues is involved in chemokine engagement, a structure-based mutational analysis of EVM1 was employed. Mapping of the mutational results onto the surface of EVM1 reveals that a cluster of five residues (I173, S171, S134, N136, and Y69) emanating from one beta sheet is critical for CCL2 and CCL3 sequestration. Additionally, we find that the extended beta2-beta4 loop flanking this conserved cluster is also essential for high-affinity, lasting interactions with chemokines. This analysis provides insight into the mechanism of CC-chemokine inhibition employed by the poxvirus family of chemokine decoy receptors.
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(as it appears on PubMed at http://www.pubmed.gov), where 16840324 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16840324}}
==About this Structure==
==About this Structure==
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[[Category: Immune system]]
[[Category: Immune system]]
[[Category: Viral protein]]
[[Category: Viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:26:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:52:38 2008''

Revision as of 21:52, 27 July 2008

Image:2grk.png

Template:STRUCTURE 2grk

Crystal structure of ectromelia virus EVM1 chemokine binding protein

Template:ABSTRACT PUBMED 16840324

About this Structure

2GRK is a Single protein structure of sequence from Ectromelia virus. Full crystallographic information is available from OCA.

Reference

Structural determinants of chemokine binding by an Ectromelia virus-encoded decoy receptor., Arnold PL, Fremont DH, J Virol. 2006 Aug;80(15):7439-49. PMID:16840324

Page seeded by OCA on Mon Jul 28 00:52:38 2008

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