1w8h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1w8h.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1w8h.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1w8h| PDB=1w8h | SCENE= }}
{{STRUCTURE_1w8h| PDB=1w8h | SCENE= }}
-
'''STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LEWISA TRISACCHARIDE'''
+
===STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LEWISA TRISACCHARIDE===
-
==Overview==
+
<!--
-
One of the mechanisms contributing to the protection by breast-feeding of the newborn against enteric diseases is related to the ability of human milk oligosaccharides to prevent the attachment of pathogenic bacteria to the duodenual epithelium. Indeed, a variety of fucosylated oligosaccharides, specific to human milk, form part of the innate immune system. In the present study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas aeruginosa, by milk oligosaccharides. Two fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M respectively. Thermodynamic studies indicate that these interactions are dominated by enthalpy. The entropy contribution is slightly favourable when binding to fucose and to the highest-affinity ligand, Lewis a. The high-resolution X-ray structures of two complexes of PA-IIL with milk oligosaccharides allow the precise determination of the conformation of a trisaccharide and a pentasaccharide. The different types of interaction between the oligosaccharides and the protein involve not only hydrogen bonding, but also calcium- and water-bridged contacts, allowing a rationalization of the thermodynamic data. This study provides important structural information about compounds that could be of general application in new therapeutic strategies against bacterial infections.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15790314}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15790314 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15790314}}
==About this Structure==
==About this Structure==
Line 37: Line 41:
[[Category: Wimmerova, M.]]
[[Category: Wimmerova, M.]]
[[Category: Pseudomonas aeruginosa,lectin,sugar,lewis a,cystic fibrosis]]
[[Category: Pseudomonas aeruginosa,lectin,sugar,lewis a,cystic fibrosis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:18:09 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:53:06 2008''

Revision as of 21:53, 27 July 2008

Image:1w8h.png

Template:STRUCTURE 1w8h

STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LEWISA TRISACCHARIDE

Template:ABSTRACT PUBMED 15790314

About this Structure

1W8H is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa., Perret S, Sabin C, Dumon C, Pokorna M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerova M, Mitchell EP, Imberty A, Biochem J. 2005 Jul 15;389(Pt 2):325-32. PMID:15790314

Page seeded by OCA on Mon Jul 28 00:53:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w8h"
Personal tools