2pmd

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{{STRUCTURE_2pmd| PDB=2pmd | SCENE= }}
{{STRUCTURE_2pmd| PDB=2pmd | SCENE= }}
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'''The structures of aIF2gamma subunit from the archaeon Sulfolobus solfataricus in the GDP-bound form.'''
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===The structures of aIF2gamma subunit from the archaeon Sulfolobus solfataricus in the GDP-bound form.===
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==Overview==
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Heterotrimeric a/eIF2alphabetagamma (archaeal homologue of the eukaryotic translation initiation factor 2 with alpha, beta and gamma subunits) delivers charged initiator tRNA (tRNAi) to the small ribosomal subunit. In this work, we determined the structures of aIF2gamma from the archaeon Sulfolobus solfataricus in the nucleotide-free and GDP-bound forms. Comparison of the free, GDP and Gpp(NH)p-Mg2+ forms of aIF2gamma revealed a sequence of conformational changes upon GDP and GTP binding. Our results show that the affinity of GDP to the G domain of the gamma subunit is higher than that of Gpp(NH)p. In analyzing a pyrophosphate molecule binding to domain II of the gamma subunit, we found a cleft that is very suitable for the acceptor stem of tRNA accommodation. It allows the suggestion of an alternative position for Met-tRNA i Met on the alphagamma intersubunit dimer, at variance with a recently published one. In the model reported here, the acceptor stem of the tRNAi is approximately perpendicular to that of tRNA in the ternary complex elongation factor Tu-Gpp(NH)p-tRNA. According to our analysis, the elbow and T stem of Met-tRNA i Met in this position should make extensive contact with the alpha subunit of aIF2. Thus, this model is in good agreement with experimental data showing that the alpha subunit of aIF2 is necessary for the stable interaction of aIF2gamma with Met-tRNA i Met.
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(as it appears on PubMed at http://www.pubmed.gov), where 17825838 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17825838}}
==About this Structure==
==About this Structure==
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[[Category: Nucleotide binding]]
[[Category: Nucleotide binding]]
[[Category: Pyrophosphate]]
[[Category: Pyrophosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:58:59 2008''

Revision as of 21:59, 27 July 2008

Image:2pmd.png

Template:STRUCTURE 2pmd

The structures of aIF2gamma subunit from the archaeon Sulfolobus solfataricus in the GDP-bound form.

Template:ABSTRACT PUBMED 17825838

About this Structure

2PMD is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

New insights into the interactions of the translation initiation factor 2 from archaea with guanine nucleotides and initiator tRNA., Nikonov O, Stolboushkina E, Nikulin A, Hasenohrl D, Blasi U, Manstein DJ, Fedorov R, Garber M, Nikonov S, J Mol Biol. 2007 Oct 19;373(2):328-36. Epub 2007 Aug 2. PMID:17825838

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