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1xf7

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{{STRUCTURE_1xf7| PDB=1xf7 | SCENE= }}
{{STRUCTURE_1xf7| PDB=1xf7 | SCENE= }}
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'''High Resolution NMR Structure of the Wilms' Tumor Suppressor Protein (WT1) Finger 3'''
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===High Resolution NMR Structure of the Wilms' Tumor Suppressor Protein (WT1) Finger 3===
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==Overview==
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The zinc finger, a motif of protein-nucleic acid recognition broadly conserved among eukaryotes, is a globular minidomain containing a tetrahedral metal-binding site. Preferential coordination of Zn(2+) (relative to Co(2+)) is proposed to reflect differences in ligand-field stabilization energies (LFSEs) due to complete or incomplete occupancy of d orbitals. LFSE predicts that the preference for Zn(2+) should be purely enthalpic in accord with calorimetric studies of a high-affinity consensus peptide (CP-1; Blasie, C. A., and Berg, J. (2002) Biochemistry 41, 15068-73). Despite its elegance, the general predominance of LFSE is unclear as (i) the magnitude by which CP-1 prefers Zn(2+) is greater than that expected and (ii) the analogous metal ion selectivity of a zinc metalloenzyme (carbonic anhydrase) is driven by changes in entropy rather than enthalpy. Because CP-1 was designed to optimize zinc binding, we have investigated the NMR structure and metal ion selectivity of a natural finger of lower stability derived from human tumor-suppressor protein WT1. Raman spectroscopy suggests that the structure of the WT1 domain is unaffected by interchange of Zn(2+) and Co(2+). As in CP-1, preferential binding of Zn(2+) (relative to Co(2+)) is driven predominantly by differences in enthalpy, but in this case the enthalpic advantage is less than that predicted by LFSE. A theoretical framework is presented to define the relationship between LFSE and other thermodynamic factors, such as metal ion electroaffinities, enthalpies of hydration, and the topography of the underlying folding landscape. The contribution of environmental coupling to entropy-enthalpy compensation is delineated in a formal thermodynamic cycle. Together, these considerations indicate that LFSE provides an important but incomplete description of the stringency and thermodynamic origin of metal-ion selectivity.
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(as it appears on PubMed at http://www.pubmed.gov), where 15518539 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15518539}}
==About this Structure==
==About this Structure==
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1XF7 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XF7 OCA].
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1XF7 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XF7 OCA].
==Reference==
==Reference==
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[[Category: Weiss, M A.]]
[[Category: Weiss, M A.]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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Revision as of 23:46, 27 July 2008

Image:1xf7.png

Template:STRUCTURE 1xf7

High Resolution NMR Structure of the Wilms' Tumor Suppressor Protein (WT1) Finger 3

Template:ABSTRACT PUBMED 15518539

About this Structure

1XF7 is a Single protein structure. Full experimental information is available from OCA.

Reference

Why zinc fingers prefer zinc: ligand-field symmetry and the hidden thermodynamics of metal ion selectivity., Lachenmann MJ, Ladbury JE, Dong J, Huang K, Carey P, Weiss MA, Biochemistry. 2004 Nov 9;43(44):13910-25. PMID:15518539

Page seeded by OCA on Mon Jul 28 02:46:04 2008

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