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2f4f

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[[Image:2f4f.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2f4f| PDB=2f4f | SCENE= }}
{{STRUCTURE_2f4f| PDB=2f4f | SCENE= }}
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'''Crystal structure of IS200 transposase'''
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===Crystal structure of IS200 transposase===
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==Overview==
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IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by exchanging the C-terminal alpha-helix and by merging the two central beta-sheets into a large beta-sheet. Glu(55), His(62), and four water molecules provide the direct coordination sphere of the catalytically essential metal ion in the Mn(2+)-bound structure. His(16), Asp(59), and His(60) also play important roles in maintaining the metal binding site. The catalytic site is formed at the interface between monomers. The candidate nucleophile in the transposition mechanism, strictly conserved Tyr(121) coming from the other monomer, is turned away from the active site, suggesting that a conformational change is likely to occur during the catalytic cycle.
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The line below this paragraph, {{ABSTRACT_PUBMED_16340015}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16340015 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16340015}}
==About this Structure==
==About this Structure==
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[[Category: Yoon, J Y.]]
[[Category: Yoon, J Y.]]
[[Category: Mn complex]]
[[Category: Mn complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:26:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:51:25 2008''

Revision as of 23:51, 27 July 2008

Image:2f4f.png

Template:STRUCTURE 2f4f

Crystal structure of IS200 transposase

Template:ABSTRACT PUBMED 16340015

About this Structure

2F4F is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Crystal structure of a metal ion-bound IS200 transposase., Lee HH, Yoon JY, Kim HS, Kang JY, Kim KH, Kim DJ, Ha JY, Mikami B, Yoon HJ, Suh SW, J Biol Chem. 2006 Feb 17;281(7):4261-6. Epub 2005 Dec 11. PMID:16340015

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