From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1sap.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1sap.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1sap| PDB=1sap | SCENE= }} | | {{STRUCTURE_1sap| PDB=1sap | SCENE= }} |
| | | | |
| - | '''HYPERTHERMOPHILE PROTEIN, RELAXATION MATRIX REFINEMENT STRUCTURE'''
| + | ===HYPERTHERMOPHILE PROTEIN, RELAXATION MATRIX REFINEMENT STRUCTURE=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius are a heterogeneous mixture of small, thermostable, nonspecific DNA-binding proteins. One of these proteins, Sac7d, has been overexpressed in Escherichia coli to provide a homogeneous preparation for structure, stability, and function studies. We present here essentially complete sequence-specific 1H NMR assignments for Sac7d, a delineation of secondary structural elements, and the high-resolution solution structure obtained from a full relaxation matrix refinement. The final structure provides an excellent fit to the NMR data with an NOE R-factor of 0.27 for backbone NOEs. The structure has a compact globular fold with 82% of the sequence involved in regular secondary structure: an antiparallel two-stranded beta-ribbon with a tight turn, followed by a short 3(10) helix, an antiparallel three-stranded beta-sheet, another short 3(10) helix, and finally four turns of alpha-helix. The amphipathic alpha-helix packs across the hydrophobic face of the three-stranded beta-sheet in an open-faced sandwich arrangement with at least one turn of the helix exposed beyond the sheet. The hydrophobic face of the beta-ribbon packs against a corner of the twisted beta-sheet. The single tryptophan responsible for the 88% fluorescence quenching upon DNA binding is exposed on the surface of the three-stranded beta-sheet. Lysines 5 and 7, whose monomethylation may be associated with enhanced thermostability, are highly solvent exposed along the inner edge of the two-stranded ribbon. The structure of Sac7d differs in many respects from that reported for the homologous native Sso7d [Baumann et al. (1994) Nature Struct. Biol. 1, 808] with a backbone RMSD greater than 3.0 A, largely due to the packing and length of the C-terminal alpha-helix which may be important in Sac7d DNA binding. | + | The line below this paragraph, {{ABSTRACT_PUBMED_7577913}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7577913 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_7577913}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | 1SAP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAP OCA]. | + | 1SAP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAP OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 25: |
Line 29: |
| | [[Category: Shriver, J W.]] | | [[Category: Shriver, J W.]] |
| | [[Category: Dna-binding protein]] | | [[Category: Dna-binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:29:18 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:05:13 2008'' |
Revision as of 01:05, 28 July 2008
Template:STRUCTURE 1sap
HYPERTHERMOPHILE PROTEIN, RELAXATION MATRIX REFINEMENT STRUCTURE
Template:ABSTRACT PUBMED 7577913
About this Structure
1SAP is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full experimental information is available from OCA.
Reference
Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius., Edmondson SP, Qiu L, Shriver JW, Biochemistry. 1995 Oct 17;34(41):13289-304. PMID:7577913
Page seeded by OCA on Mon Jul 28 04:05:13 2008
