2g7f

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{{STRUCTURE_2g7f| PDB=2g7f | SCENE= }}
{{STRUCTURE_2g7f| PDB=2g7f | SCENE= }}
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'''The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I'''
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===The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I===
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==Overview==
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The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted to 1.6 A (on BM01A at the ESRF) and 1.95 A (on a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme at different pH values are essentially identical to each other and to the V. vulnificus enzyme. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is present in the structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH.
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{{ABSTRACT_PUBMED_17057343}}
==About this Structure==
==About this Structure==
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[[Category: Endonuclease i]]
[[Category: Endonuclease i]]
[[Category: Vibrio cholerae nuclease]]
[[Category: Vibrio cholerae nuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:46:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:23:25 2008''

Revision as of 01:23, 28 July 2008

Image:2g7f.png

Template:STRUCTURE 2g7f

The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I

Template:ABSTRACT PUBMED 17057343

About this Structure

2G7F is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion., Altermark B, Smalas AO, Willassen NP, Helland R, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1387-91. Epub 2006, Oct 18. PMID:17057343

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