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2poj

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{{STRUCTURE_2poj| PDB=2poj | SCENE= }}
{{STRUCTURE_2poj| PDB=2poj | SCENE= }}
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'''NMR Solution Structure of the Inhibitor-Free State of Macrophage Metalloelastase (MMP-12)'''
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===NMR Solution Structure of the Inhibitor-Free State of Macrophage Metalloelastase (MMP-12)===
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==Overview==
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Macrophage metalloelastase or matrix metalloproteinase-12 (MMP-12) appears to exacerbate atherosclerosis, emphysema, aortic aneurysm, rheumatoid arthritis, and inflammatory bowel disease. An inactivating E219A mutation, validated by crystallography and NMR spectra, prevents autolysis of MMP-12 and allows us to determine its NMR structure without an inhibitor. The structural ensemble of the catalytic domain without an inhibitor is based on 2813 nuclear Overhauser effects (NOEs) and has an average RMSD to the mean structure of 0.25 A for the backbone and 0.61 A for all heavy atoms for residues Trp109-Gly263. Compared to crystal structures of MMP-12, helix B (hB) at the active site is unexpectedly more deeply recessed under the beta-sheet. This opens a pocket between hB and beta-strand IV in the active-site cleft. Both hB and an internal cavity are shifted toward beta-strand I, beta-strand III, and helix A on the back side of the protease. About 25 internal NOE contacts distinguish the inhibitor-free solution structure and indicate hB's greater depth and proximity to the sheet and helix A. Line broadening and multiplicity of amide proton NMR peaks from hB are consistent with hB undergoing a slow conformational exchange among subtly different environments. Inhibitor-binding-induced perturbations of the NMR spectra of MMP-1 and MMP-3 map to similar locations across MMP-12 and encompass the internal conformational adjustments. Evolutionary trace analysis suggests a functionally important network of residues that encompasses most of the locations adjusting in conformation, including 18 residues with NOE contacts unique to inhibitor-free MMP-12. The conformational change, sequence analysis, and inhibitor perturbations of NMR spectra agree on the network they identify between structural scaffold and the active site of MMPs.
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(as it appears on PubMed at http://www.pubmed.gov), where 17997411 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17997411}}
==About this Structure==
==About this Structure==
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2POJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2POJ OCA].
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2POJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2POJ OCA].
==Reference==
==Reference==
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[[Category: Matrix metaloproteinase]]
[[Category: Matrix metaloproteinase]]
[[Category: Mmp-12]]
[[Category: Mmp-12]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:32:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:25:22 2008''

Revision as of 01:25, 28 July 2008

Image:2poj.png

Template:STRUCTURE 2poj

NMR Solution Structure of the Inhibitor-Free State of Macrophage Metalloelastase (MMP-12)

Template:ABSTRACT PUBMED 17997411

About this Structure

2POJ is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of inhibitor-free human metalloelastase (MMP-12) indicates an internal conformational adjustment., Bhaskaran R, Palmier MO, Bagegni NA, Liang X, Van Doren SR, J Mol Biol. 2007 Dec 14;374(5):1333-44. Epub 2007 Oct 16. PMID:17997411

Page seeded by OCA on Mon Jul 28 04:25:22 2008

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