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| | {{STRUCTURE_1tff| PDB=1tff | SCENE= }} | | {{STRUCTURE_1tff| PDB=1tff | SCENE= }} |
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| - | '''Structure of Otubain-2'''
| + | ===Structure of Otubain-2=== |
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| - | ==Overview==
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| - | Ubiquitylation, the modification of cellular proteins by the covalent attachment of ubiquitin, is critical for diverse biological processes including cell cycle progression, signal transduction and stress response. This process can be reversed and regulated by a group of proteases called deubiquitylating enzymes (DUBs). Otubains are a recently identified family of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins. Here, we report the first crystal structure of an OTU superfamily protein, otubain 2, at 2.1 A resolution and propose a model for otubain-ubiquitin binding on the basis of other DUB structures. Although otubain 2 is a member of the cysteine protease superfamily of folds, its crystal structure shows a novel fold for DUBs. Moreover, the active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides, rather than the composite backbone/side-chain substructures seen in other DUBs and cysteine proteases. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15258613}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15258613 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15258613}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Tcherniuk, S O.]] | | [[Category: Tcherniuk, S O.]] |
| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:53:14 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:26:31 2008'' |
Revision as of 01:26, 28 July 2008
Template:STRUCTURE 1tff
Structure of Otubain-2
Template:ABSTRACT PUBMED 15258613
About this Structure
1TFF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human otubain 2., Nanao MH, Tcherniuk SO, Chroboczek J, Dideberg O, Dessen A, Balakirev MY, EMBO Rep. 2004 Aug;5(8):783-8. Epub 2004 Jul 16. PMID:15258613
Page seeded by OCA on Mon Jul 28 04:26:31 2008
