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2glo

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[[Image:2glo.gif|left|200px]]
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[[Image:2glo.png|left|200px]]
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{{STRUCTURE_2glo| PDB=2glo | SCENE= }}
{{STRUCTURE_2glo| PDB=2glo | SCENE= }}
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'''Solution structure of the Brinker DNA binding domain in complex with the omb enhancer'''
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===Solution structure of the Brinker DNA binding domain in complex with the omb enhancer===
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==Overview==
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The Brinker (Brk) nuclear repressor is a major element of the Drosophila Decapentaplegic morphogen signaling pathway. Its N-terminal part has weak homology to the Antennapedia homeodomain and binds to GC-rich DNA sequences. We have investigated the conformation and dynamics of the N-terminal 101 amino acid residues of Brk in the absence and in the presence of cognate DNA by solution NMR spectroscopy. In the absence of DNA, Brk is unfolded and highly flexible throughout the entire backbone. Addition of cognate DNA induces the formation of a well-folded structure for residues R46 to R95. This structure consists of four helices forming a helix-turn-helix motif that differs from homeodomains, but has similarities to the Tc3 transposase, the Pax-6 Paired domain, and the human centromere-binding protein. The GC-rich DNA recognition can be explained by specific major groove hydrogen bonds from the N-terminal end of helix alpha3. The transition from a highly flexible, completely unfolded conformation in the absence of DNA to a well-formed structure in the complex presents a very extreme case of the "coupling of binding and folding" phenomenon.
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The line below this paragraph, {{ABSTRACT_PUBMED_16876822}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16876822 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16876822}}
==About this Structure==
==About this Structure==
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2GLO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLO OCA].
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2GLO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GLO OCA].
==Reference==
==Reference==
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[[Category: Helix-turn-helix motif]]
[[Category: Helix-turn-helix motif]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:14:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:20:34 2008''

Revision as of 02:20, 28 July 2008

Image:2glo.png

Template:STRUCTURE 2glo

Solution structure of the Brinker DNA binding domain in complex with the omb enhancer

Template:ABSTRACT PUBMED 16876822

About this Structure

2GLO is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

DNA recognition by the brinker repressor--an extreme case of coupling between binding and folding., Cordier F, Hartmann B, Rogowski M, Affolter M, Grzesiek S, J Mol Biol. 2006 Aug 25;361(4):659-72. Epub 2006 Jul 7. PMID:16876822

Page seeded by OCA on Mon Jul 28 05:20:34 2008

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