2z2e

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{{STRUCTURE_2z2e| PDB=2z2e | SCENE= }}
{{STRUCTURE_2z2e| PDB=2z2e | SCENE= }}
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'''Crystal Structure of Canine Milk Lysozyme Stabilized against Non-enzymatic Deamidation'''
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===Crystal Structure of Canine Milk Lysozyme Stabilized against Non-enzymatic Deamidation===
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==Overview==
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Asparaginyl deamidation is a common form of nonenzymatic degradation of proteins and peptides. As it introduces a negative charge spontaneously and irreversibly, charge heterogeneity can be accumulated in protein solution during purification, preservation, and experiments. In this study, canine milk lysozyme (CML), a useful model for the study of the molten globule state, exhibited charge heterogeneity after sample purification. Four Asn residues in CML deamidated rapidly under mild conditions: pH 8.0 and 30 degrees C. Other than these residues, one Asn residue, which was stable in the native state, was labile to deamidation in the unfolded state. This suggests that the structural formation around Asn can suppress deamidation. Substitutions of these labile Asn residues to Gln residues prevented deamidation effectively. Because the substitutions did not disrupt the structural formation of the native and molten globule states, they will enable more precise analyses for physical and structural studies. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
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{{ABSTRACT_PUBMED_18214981}}
==About this Structure==
==About this Structure==
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[[Category: Lysozyme c]]
[[Category: Lysozyme c]]
[[Category: Milk lysozyme]]
[[Category: Milk lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:36:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:22:06 2008''

Revision as of 02:22, 28 July 2008

Image:2z2e.png

Template:STRUCTURE 2z2e

Crystal Structure of Canine Milk Lysozyme Stabilized against Non-enzymatic Deamidation

Template:ABSTRACT PUBMED 18214981

About this Structure

2Z2E is a Single protein structure of sequence from Canis lupus familiaris. Full crystallographic information is available from OCA.

Reference

Spontaneous asparaginyl deamidation of canine milk lysozyme under mild conditions., Nonaka Y, Aizawa T, Akieda D, Yasui M, Watanabe M, Watanabe N, Tanaka I, Kamiya M, Mizuguchi M, Demura M, Kawano K, Proteins. 2008 Jan 23;72(1):313-322. PMID:18214981

Page seeded by OCA on Mon Jul 28 05:22:06 2008

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