This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1rxi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rxi.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1rxi.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rxi| PDB=1rxi | SCENE= }}
{{STRUCTURE_1rxi| PDB=1rxi | SCENE= }}
-
'''pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S'''
+
===pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S===
-
==Overview==
+
<!--
-
Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15159594}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15159594 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15159594}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Wyns, L.]]
[[Category: Wyns, L.]]
[[Category: Arsc]]
[[Category: Arsc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:01:32 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:28:02 2008''

Revision as of 02:28, 28 July 2008

Image:1rxi.png

Template:STRUCTURE 1rxi

pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S

Template:ABSTRACT PUBMED 15159594

About this Structure

1RXI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct., Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1180-4. Epub 2004, May 21. PMID:15159594

Page seeded by OCA on Mon Jul 28 05:28:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rxi"
Personal tools