1mqk

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{{STRUCTURE_1mqk| PDB=1mqk | SCENE= }}
{{STRUCTURE_1mqk| PDB=1mqk | SCENE= }}
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'''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2'''
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===Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2===
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==Overview==
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The antibody Fv fragment 7E2 has previously been employed in the induced crystallization of the integral membrane protein cytochrome c oxidase from Paracoccus denitrificans. The 1.3 A X-ray structure of the uncomplexed antibody fragment reveals conserved water networks on the surfaces of the framework regions. A novel consensus motif for water coordination, XX(S/T), is found along the edges of the beta-sandwich, where a water molecule forms hydrogen bonds to the carbonyl O atom of a residue at position N and the OG hydroxyl groups of conserved serines or threonines at position N + 2. Multiple conformations were found in the hydrophobic core for residues IleL21, LeuL33 and the disulfide bridges. An internal water molecule that is compatible with only one of the three packing states of the V(L) core suggests local 'breathing' of the variable domain. TrpH47, a conserved key residue of the V(H)/V(L) interface, is crucially involved in the formation of the antigen-binding site by adopting a novel conformation that specifically stabilizes the non-canonical CDR-L3 loop. Finally, a comparison with 7E2-cytochrome c oxidase complexes demonstrates that binding of this membrane-bound antigen proceeds without major conformational changes of the 7E2 antibody fragment.
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(as it appears on PubMed at http://www.pubmed.gov), where 12657787 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12657787}}
==About this Structure==
==About this Structure==
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[[Category: High-resolution structure]]
[[Category: High-resolution structure]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:36:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:43:02 2008''

Revision as of 02:43, 28 July 2008

Image:1mqk.png

Template:STRUCTURE 1mqk

Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2

Template:ABSTRACT PUBMED 12657787

About this Structure

1MQK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization., Essen LO, Harrenga A, Ostermeier C, Michel H, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):677-87. Epub 2003, Mar 25. PMID:12657787

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