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2pua

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[[Image:2pua.gif|left|200px]]
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{{STRUCTURE_2pua| PDB=2pua | SCENE= }}
{{STRUCTURE_2pua| PDB=2pua | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES'''
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===CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES===
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==Overview==
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The three-dimensional structure of a ternary complex of the purine repressor, PurR, bound to both its corepressor, hypoxanthine, and the 16-base pair purF operator site has been solved at 2.7 A resolution by x-ray crystallography. The bipartite structure of PurR consists of an amino-terminal DNA-binding domain and a larger carboxyl-terminal corepressor binding and dimerization domain that is similar to that of the bacterial periplasmic binding proteins. The DNA-binding domain contains a helix-turn-helix motif that makes base-specific contacts in the major groove of the DNA. Base contacts are also made by residues of symmetry-related alpha helices, the "hinge" helices, which bind deeply in the minor groove. Critical to hinge helix-minor groove binding is the intercalation of the side chains of Leu54 and its symmetry-related mate, Leu54', into the central CpG-base pair step. These residues thereby act as "leucine levers" to pry open the minor groove and kink the purF operator by 45 degrees.
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(as it appears on PubMed at http://www.pubmed.gov), where 7973627 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7973627}}
==About this Structure==
==About this Structure==
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[[Category: Zalkin, H.]]
[[Category: Zalkin, H.]]
[[Category: Dna-binding regulatory protein]]
[[Category: Dna-binding regulatory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:48:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:53:22 2008''

Revision as of 02:53, 28 July 2008

Image:2pua.png

Template:STRUCTURE 2pua

CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES

Template:ABSTRACT PUBMED 7973627

About this Structure

2PUA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices., Schumacher MA, Choi KY, Zalkin H, Brennan RG, Science. 1994 Nov 4;266(5186):763-70. PMID:7973627

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