This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1yua

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1yua.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1yua.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1yua| PDB=1yua | SCENE= }}
{{STRUCTURE_1yua| PDB=1yua | SCENE= }}
-
'''C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I'''
+
===C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I===
-
==Overview==
+
<!--
-
Escherichia coli DNA topoisomerase I catalyzes the interconversion of different topological forms of DNA. In this paper we describe NMR studies of a 14K C-terminal fragment of this enzyme that binds preferentially to single-stranded DNA and enhances the enzyme's ability to relax negatively supercoiled DNA under high salt conditions. The 1H, 13C, and 15N resonances of the protein were assigned from a number of heteronuclear multidimensional NMR experiments, and the three-dimensional structure of the protein was determined from a total of 2188 NMR-derived restraints. The root-mean-square deviation about the mean coordinate positions for residues 13-120 is 0.68 +/- 0.11 A for the backbone atoms and 1.09 +/- 0.09 A for all heavy atoms. The overall fold, which consists of two four-stranded beta-sheets separated by two helices, differs from other DNA- and RNA-binding proteins such as gene 5, cold shock protein, and hnRNP C. From an analysis of the changes in chemical shift upon the addition of single-stranded DNA, the location of the oligonucleotide binding site was determined. The binding site consists of a beta-sheet containing positively charged and aromatic amino acids and, in spite of its different structure, is similar to that found in other proteins that bind single-stranded oligonucleotides.
+
The line below this paragraph, {{ABSTRACT_PUBMED_7779808}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 7779808 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_7779808}}
==About this Structure==
==About this Structure==
-
1YUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUA OCA].
+
1YUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUA OCA].
==Reference==
==Reference==
Line 28: Line 32:
[[Category: Zhu, C X.]]
[[Category: Zhu, C X.]]
[[Category: Gene-regulating protein]]
[[Category: Gene-regulating protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:47:52 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:57:16 2008''

Revision as of 02:57, 28 July 2008

Image:1yua.png

Template:STRUCTURE 1yua

C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I

Template:ABSTRACT PUBMED 7779808

About this Structure

1YUA is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure of the C-terminal single-stranded DNA-binding domain of Escherichia coli topoisomerase I., Yu L, Zhu CX, Tse-Dinh YC, Fesik SW, Biochemistry. 1995 Jun 13;34(23):7622-8. PMID:7779808

Page seeded by OCA on Mon Jul 28 05:57:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1yua"
Personal tools