This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2jx3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2jx3.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2jx3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2jx3| PDB=2jx3 | SCENE= }}
{{STRUCTURE_2jx3| PDB=2jx3 | SCENE= }}
-
'''NMR solution structure of the N-terminal domain of DEK'''
+
===NMR solution structure of the N-terminal domain of DEK===
-
==Overview==
+
<!--
-
The human DEK protein has a long-standing association with carcinogenesis since the DEK gene was originally identified in the t(6:9) chromosomal translocation in a subtype of patients with acute myelogenous leukemia (AML). Recent studies have partly unveiled DEK's cellular functions including apoptosis inhibition in primary cells as well as cancer cells, determination of 3' splice site of transcribed RNA, and suppression of transcription initiation by polymerase II. It has been previously shown that the N-terminal region of DEK, spanning residues 68-226, confers important in vitro and in vivo functions of DEK, which include double-stranded DNA (ds-DNA) binding, introduction of constrained positive supercoils into closed dsDNA, and apoptosis inhibition. In this paper, we describe the three-dimensional structure of the N-terminal domain of DEK (DEKntd) as determined using solution NMR. The C-terminal part of DEKntd, which contains a putative DNA-binding motif (SAF/SAP motif), folds into a helix-loop-helix structure. Interestingly, the N-terminal part of DEKntd shows a very similar structure to the C-terminal part, although the N-terminal and the C-terminal part differ distinctively in their amino acid sequences. As a consequence, the structure of DEKntd has a pseudo twofold plane symmetry. In addition, we tested dsDNA binding of DEKntd by monitoring changes of NMR chemical shifts upon addition of dsDNAs. We found that not only the C-terminal part containing the SAF/SAP motif but the N-terminal part is also involved in DEKntd's dsDNA binding. Our study illustrates a new structural variant and reveals novel dsDNA-binding properties for proteins containing the SAP/SAF motif.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18227428}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18227428 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18227428}}
==Disease==
==Disease==
Line 19: Line 23:
==About this Structure==
==About this Structure==
-
2JX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX3 OCA].
+
2JX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX3 OCA].
==Reference==
==Reference==
Line 36: Line 40:
[[Category: Proto-oncogene]]
[[Category: Proto-oncogene]]
[[Category: Saf/sap motif]]
[[Category: Saf/sap motif]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:22 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:12:21 2008''

Revision as of 03:12, 28 July 2008

Image:2jx3.png

Template:STRUCTURE 2jx3

Contents

NMR solution structure of the N-terminal domain of DEK

Template:ABSTRACT PUBMED 18227428

Disease

Known disease associated with this structure: Leukemia, acute nonlymphocytic OMIM:[125264]

About this Structure

2JX3 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution NMR structure of the N-terminal domain of the human DEK protein., Devany M, Kappes F, Chen KM, Markovitz DM, Matsuo H, Protein Sci. 2008 Feb;17(2):205-15. PMID:18227428

Page seeded by OCA on Mon Jul 28 06:12:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jx3"
Personal tools