1qml

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{{STRUCTURE_1qml| PDB=1qml | SCENE= }}
{{STRUCTURE_1qml| PDB=1qml | SCENE= }}
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'''HG COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE'''
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===HG COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE===
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==Overview==
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MAD experiments attempting to solve the structure of 5--aminolaevulinic acid dehydratase using Zn and Pb edges are described. The data obtained proved insufficient for a complete structure solution but were invaluable in subsequent identification of metal-binding sites using anomalous difference Fourier analyses once the structure of the enzyme had been solved. These sites include the highly inhibitory substitution of an enzymic cofactor Zn(2+) ion by Pb(2+) ions, which represents a major contribution towards understanding the molecular basis of lead poisoning. The MAD data collected at the Pb edge were also used with isomorphous replacement data from the same Pb co-crystal and a Hg co-crystal to provide the first delineation of the enzyme's quaternary structure. In this MADIR analysis, the Hg co-crystal data were treated as native data. Anomalous difference Fouriers were again used, revealing that Hg(2+) had substituted for the same Zn(2+) cofactor ion as had Pb(2+), a finding of fundamental importance for the understanding of mercury poisoning. In addition, Pt(2+) ions were found to bind at the same place in the structure. The refined structures of the Pb- and the Hg-complexed enzymes are presented at 2.5 and 3.0 A resolution, respectively.
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(as it appears on PubMed at http://www.pubmed.gov), where 10739915 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10739915}}
==About this Structure==
==About this Structure==
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[[Category: Tetrapyrrole synthesis]]
[[Category: Tetrapyrrole synthesis]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:27:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:50:27 2008''

Revision as of 03:50, 28 July 2008

Image:1qml.png

Template:STRUCTURE 1qml

HG COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE

Template:ABSTRACT PUBMED 10739915

About this Structure

1QML is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

MAD analyses of yeast 5-aminolaevulinate dehydratase: their use in structure determination and in defining the metal-binding sites., Erskine PT, Duke EM, Tickle IJ, Senior NM, Warren MJ, Cooper JB, Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):421-30. PMID:10739915

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