1vjl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1vjl.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1vjl.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1vjl| PDB=1vjl | SCENE= }}
{{STRUCTURE_1vjl| PDB=1vjl | SCENE= }}
-
'''CRYSTAL STRUCTURE OF PREDICTED PROTEIN RELATED TO WOUND INDUCIVE PROTEINS IN PLANTS (TM0160) FROM THERMOTOGA MARITIMA AT 1.90 A RESOLUTION'''
+
===CRYSTAL STRUCTURE OF PREDICTED PROTEIN RELATED TO WOUND INDUCIVE PROTEINS IN PLANTS (TM0160) FROM THERMOTOGA MARITIMA AT 1.90 A RESOLUTION===
-
==Overview==
+
<!--
-
The structure of two Thermotoga maritima proteins, a conserved hypothetical protein (TM0160) and a transcriptional regulator (TM1171), have now been determined at 1.9 A and 2.3 A resolution, respectively, as part of a large-scale structural genomics project. Our first efforts to crystallize full-length versions of these targets were unsuccessful. However, analysis of the recombinant purified proteins using the technique of enhanced amide hydrogen/deuterium exchange mass spectroscopy (DXMS) revealed substantial regions of rapid amide deuterium hydrogen exchange, consistent with flexible regions of the structures. Based on these exchange data, truncations were designed to selectively remove the disordered C-terminal regions, and the resulting daughter proteins showed greatly enhanced crystallizability. Comparative DXMS analysis of full-length protein versus truncated forms demonstrated complete and exact preservation of the exchange rate profiles in the retained sequence, indicative of conservation of the native folded structure. This study presents the first structures produced with the aid of the DXMS method for salvaging intractable crystallization targets. The structure of TM0160 represents a new fold and highlights the use of this approach where any prior structural knowledge is absent. The structure of TM1171 represents an example where the lack of a substrate/cofactor may impair crystallization. The details of both structures are presented and discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15557262}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15557262 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15557262}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Tm0160]]
[[Category: Tm0160]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:36:19 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:09:46 2008''

Revision as of 04:09, 28 July 2008

Image:1vjl.png

Template:STRUCTURE 1vjl

CRYSTAL STRUCTURE OF PREDICTED PROTEIN RELATED TO WOUND INDUCIVE PROTEINS IN PLANTS (TM0160) FROM THERMOTOGA MARITIMA AT 1.90 A RESOLUTION

Template:ABSTRACT PUBMED 15557262

About this Structure

1VJL is a Single protein structure of sequence from Thermotoga maritima. This structure supersedes the now removed PDB entry 1o5y. Full crystallographic information is available from OCA.

Reference

On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171., Spraggon G, Pantazatos D, Klock HE, Wilson IA, Woods VL Jr, Lesley SA, Protein Sci. 2004 Dec;13(12):3187-99. PMID:15557262

Page seeded by OCA on Mon Jul 28 07:09:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vjl"
Personal tools