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2du9

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{{STRUCTURE_2du9| PDB=2du9 | SCENE= }}
{{STRUCTURE_2du9| PDB=2du9 | SCENE= }}
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'''crystal structure of the transcriptional factor from C.glutamicum'''
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===crystal structure of the transcriptional factor from C.glutamicum===
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==Overview==
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Among the transcription factors, the helix-turn-helix (HTH) GntR family comprised of FadR, HutC, MocR, YtrA, AraR, and PlmA subfamilies regulates the most varied biological processes. Generally, proteins belonging to this family contain an N-terminal DNA-binding domain and a C-terminal effector-binding/oligomerization domain. The members of the YtrA subfamily are much shorter than other members of this family, with chain lengths of 120-130 residues with about 50 residues located in the C-terminal domain. Because of this length, the mode of dimerization and the ability to bind effectors by the C-terminal domain are puzzling. Here, we first report the structure of the transcription factor CGL2947 from Corynebacterium glutamicum, which belongs to the YtrA family. The monomer is composed of a DNA-binding domain containing a winged HTH motif in the N terminus and two helices (alpha4 and alpha5) with a fishhook-shaped arrangement in the C terminus. Helices alpha4 and alpha5 of two monomers intertwine together to form a novel homodimer assembly. The effector-accommodating pocket with 2-methyl-2,4-pentanediol (MPD) docked was located, and it was suggested to represent a novel mode of effector binding. The structures in two crystal forms (MPD-free and -bound in the proposed effector-binding pocket) were solved. The structural variations have implications regarding how the effector-induced conformational change modulates DNA affinity for YtrA family members.
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{{ABSTRACT_PUBMED_17766384}}
==About this Structure==
==About this Structure==
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[[Category: Yao, M.]]
[[Category: Yao, M.]]
[[Category: Winged helix-turn-helix]]
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Revision as of 04:35, 28 July 2008

Image:2du9.png

Template:STRUCTURE 2du9

crystal structure of the transcriptional factor from C.glutamicum

Template:ABSTRACT PUBMED 17766384

About this Structure

2DU9 is a Single protein structure of sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA.

Reference

The structures of transcription factor CGL2947 from Corynebacterium glutamicum in two crystal forms: a novel homodimer assembling and the implication for effector-binding mode., Gao YG, Yao M, Itou H, Zhou Y, Tanaka I, Protein Sci. 2007 Sep;16(9):1878-86. PMID:17766384

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