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2q0o

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{{STRUCTURE_2q0o| PDB=2q0o | SCENE= }}
{{STRUCTURE_2q0o| PDB=2q0o | SCENE= }}
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'''Crystal structure of an anti-activation complex in bacterial quorum sensing'''
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===Crystal structure of an anti-activation complex in bacterial quorum sensing===
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==Overview==
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Bacteria can communicate via diffusible signal molecules they generate and release to coordinate their behavior in response to the environment. Signal molecule concentration is often proportional to bacterial population density, and when this reaches a critical concentration, reflecting a bacterial quorum, specific behaviors including virulence, symbiosis, and horizontal gene transfer are activated. Quorum-sensing regulation in many Gram-negative bacteria involves acylated homoserine lactone signals that are perceived through binding to LuxR-type, acylated-homoserine-lactone-responsive transcription factors. Bacteria of the rhizobial group employ the LuxR-type transcriptional activator TraR in quorum sensing, and its activity is further regulated through interactions with the TraM antiactivator. In this study, we have crystallographically determined the 3D structure of the TraR-TraM antiactivation complex from Rhizobium sp. strain NGR234. Unexpectedly, the antiactivator TraM binds to TraR at a site distinct from its DNA-binding motif and induces an allosteric conformational change in the protein, thereby preventing DNA binding. Structural analysis reveals a highly conserved TraR-TraM interface and suggests a mechanism for antiactivation complex formation. This structure may inform alternative strategies to control quorum-sensing-regulated microbial activity including amelioration of infectious disease and antibiotic resistance. In addition, the structural basis of antiactivation presents a regulatory interaction that provides general insights relevant to the field of transcription regulation and signal transduction.
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{{ABSTRACT_PUBMED_17921255}}
==About this Structure==
==About this Structure==
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:07:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:09:42 2008''

Revision as of 06:09, 28 July 2008

Image:2q0o.png

Template:STRUCTURE 2q0o

Crystal structure of an anti-activation complex in bacterial quorum sensing

Template:ABSTRACT PUBMED 17921255

About this Structure

2Q0O is a Protein complex structure of sequences from Rhizobium sp.. Full crystallographic information is available from OCA.

Reference

Structural basis for antiactivation in bacterial quorum sensing., Chen G, Jeffrey PD, Fuqua C, Shi Y, Chen L, Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16474-9. Epub 2007 Oct 5. PMID:17921255

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