1sa3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1sa3.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1sa3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1sa3| PDB=1sa3 | SCENE= }}
{{STRUCTURE_1sa3| PDB=1sa3 | SCENE= }}
-
'''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''
+
===An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site===
-
==Overview==
+
<!--
-
Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15341737}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15341737 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15341737}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Xu, Q S.]]
[[Category: Xu, Q S.]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:28:21 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:41:40 2008''

Revision as of 06:41, 28 July 2008

Image:1sa3.png

Template:STRUCTURE 1sa3

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site

Template:ABSTRACT PUBMED 15341737

About this Structure

1SA3 is a Single protein structure of sequence from Moraxella sp.. Full crystallographic information is available from OCA.

Reference

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:15341737

Page seeded by OCA on Mon Jul 28 09:41:40 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sa3"
Personal tools