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2akq

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{{STRUCTURE_2akq| PDB=2akq | SCENE= }}
{{STRUCTURE_2akq| PDB=2akq | SCENE= }}
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'''The structure of bovine B-lactoglobulin A in crystals grown at very low ionic strength'''
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===The structure of bovine B-lactoglobulin A in crystals grown at very low ionic strength===
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==Overview==
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Bovine beta-lactoglobulin (BLG) is a globular protein of uncertain physiological function and a member of the lipocalin superfamily of proteins. Here, we present the X-ray structure at 3.0 angstroms of BLG (variant A) from an orthorhombic (P2(1)2(1)2(1)) pseudo-tetragonal crystal form that suffers from pseudo-merohedral twinning (final R(working) = 0.224, R(free) = 0.265). Crystals were grown by dialysis against ultra-purified water (i.e., at very low ionic strength), at pH approximately 5.2 (approximately pI), conditions vastly different from all other BLG structures determined previously. This allows critical assessment of the BLG structure and of the influence that pH, ionic strength, and crystal packing may have on the molecular structure of BLG. The pH-sensitive EF loop is found in the closed conformation characteristic of BLG at pH less than 7 and moderate to high ionic strength. Although the hydrophobic pocket appears to be empty, the presence of highly disordered water molecules cannot be excluded. The dimer interface and the hydrophobic pocket (calyx) are preserved. However, the orientation of the subunits in the dimer varies considerably with crystal form. Structure is deposited with PDB ID 2akq.
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(as it appears on PubMed at http://www.pubmed.gov), where 16540345 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16540345}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of bovine beta-lactoglobulin (variant A) at very low ionic strength., Adams JJ, Anderson BF, Norris GE, Creamer LK, Jameson GB, J Struct Biol. 2006 Jun;154(3):246-54. Epub 2006 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16540345 16540345]
Structure of bovine beta-lactoglobulin (variant A) at very low ionic strength., Adams JJ, Anderson BF, Norris GE, Creamer LK, Jameson GB, J Struct Biol. 2006 Jun;154(3):246-54. Epub 2006 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16540345 16540345]
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Bovine beta-lactoglobulin at 1.8 A resolution--still an enigmatic lipocalin., Brownlow S, Morais Cabral JH, Cooper R, Flower DR, Yewdall SJ, Polikarpov I, North AC, Sawyer L, Structure. 1997 Apr 15;5(4):481-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9115437 9115437]
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Structural basis of the Tanford transition of bovine beta-lactoglobulin., Qin BY, Bewley MC, Creamer LK, Baker HM, Baker EN, Jameson GB, Biochemistry. 1998 Oct 6;37(40):14014-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9760236 9760236]
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12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin., Qin BY, Creamer LK, Baker EN, Jameson GB, FEBS Lett. 1998 Nov 6;438(3):272-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9827560 9827560]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Transport protein]]
[[Category: Transport protein]]
[[Category: X-ray]]
[[Category: X-ray]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:27:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:20:43 2008''

Revision as of 07:20, 28 July 2008

Image:2akq.png

Template:STRUCTURE 2akq

The structure of bovine B-lactoglobulin A in crystals grown at very low ionic strength

Template:ABSTRACT PUBMED 16540345

About this Structure

2AKQ is a Single protein structure of sequence from Bos taurus. This structure supersedes the now removed PDB entry 1mfh. Full crystallographic information is available from OCA.

Reference

Structure of bovine beta-lactoglobulin (variant A) at very low ionic strength., Adams JJ, Anderson BF, Norris GE, Creamer LK, Jameson GB, J Struct Biol. 2006 Jun;154(3):246-54. Epub 2006 Feb 6. PMID:16540345

Bovine beta-lactoglobulin at 1.8 A resolution--still an enigmatic lipocalin., Brownlow S, Morais Cabral JH, Cooper R, Flower DR, Yewdall SJ, Polikarpov I, North AC, Sawyer L, Structure. 1997 Apr 15;5(4):481-95. PMID:9115437

Structural basis of the Tanford transition of bovine beta-lactoglobulin., Qin BY, Bewley MC, Creamer LK, Baker HM, Baker EN, Jameson GB, Biochemistry. 1998 Oct 6;37(40):14014-23. PMID:9760236

12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin., Qin BY, Creamer LK, Baker EN, Jameson GB, FEBS Lett. 1998 Nov 6;438(3):272-8. PMID:9827560

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