1wmb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1wmb.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1wmb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1wmb| PDB=1wmb | SCENE= }}
{{STRUCTURE_1wmb| PDB=1wmb | SCENE= }}
-
'''Crystal structure of NAD dependent D-3-hydroxybutylate dehydrogenase'''
+
===Crystal structure of NAD dependent D-3-hydroxybutylate dehydrogenase===
-
==Overview==
+
<!--
-
The gene coding for d-3-hydroxybutyrate dehydrogenase (HBDH) was cloned from Pseudomonas fragi. The nucleotide sequence contained a 780 bp open reading frame encoding a 260 amino acid residue protein. The recombinant enzyme was efficiently expressed in Escherichia coli cells harboring pHBDH11 and was purified to homogeneity as judged by SDS-PAGE. The enzyme showed a strict stereospecificity to the D-enantiomer (3R-configuration) of 3-hydroxybutyrate as a substrate. Crystals of the ligand-free HBDH and of the enzyme-NAD+ complex were obtained using the hanging-drop, vapor-diffusion method. The crystal structure of the HBDH was solved by the multiwavelength anomalous diffraction method using the SeMet-substituted enzyme and was refined to 2.0 A resolution. The overall structure of P.fragi HBDH, including the catalytic tetrad of Asn114, Ser142, Tyr155, and Lys159, shows obvious relationships with other members of the short-chain dehydrogenase/reductase (SDR) family. A cacodylate anion was observed in both the ligand-free enzyme and the enzyme-NAD+ complex, and was located near the catalytic tetrad. It was shown that the cacodylate inhibited the NAD+-dependent D-3-hydroxybutyrate dehydrogenation competitively, with a Ki value of 5.6 mM. From the interactions between cacodylate and the enzyme, it is predicted that substrate specificity is achieved through the recognition of the 3-methyl and carboxyl groups of the substrate.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16325199}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16325199 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16325199}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Short chain dehydrogenase]]
[[Category: Short chain dehydrogenase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:52:18 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:38:58 2008''

Revision as of 07:39, 28 July 2008

Image:1wmb.png

Template:STRUCTURE 1wmb

Crystal structure of NAD dependent D-3-hydroxybutylate dehydrogenase

Template:ABSTRACT PUBMED 16325199

About this Structure

1WMB is a Single protein structure of sequence from Pseudomonas fragi. Full crystallographic information is available from OCA.

Reference

D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme., Ito K, Nakajima Y, Ichihara E, Ogawa K, Katayama N, Nakashima K, Yoshimoto T, J Mol Biol. 2006 Jan 27;355(4):722-33. Epub 2005 Nov 14. PMID:16325199

Page seeded by OCA on Mon Jul 28 10:38:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wmb"
Personal tools