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2ehm

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{{STRUCTURE_2ehm| PDB=2ehm | SCENE= }}
{{STRUCTURE_2ehm| PDB=2ehm | SCENE= }}
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'''HA1 subcomponent of botulinum type C progenitor toxin complexed with N-acetylgalactosamine'''
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===HA1 subcomponent of botulinum type C progenitor toxin complexed with N-acetylgalactosamine===
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==Overview==
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Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this study, using the recombinant protein fused to glutathione S-transferase, we investigated the binding specificity of the HA1 subcomponent to sugars and estimated the binding sites of HA1 based on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose. An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars. On the other hand, there is no aromatic residue at site II; thus, the interaction with galactose seems to be poor. The double mutant W176A at site I and D271F at site II has no avidity for N-acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.
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{{ABSTRACT_PUBMED_18178224}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]
Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]
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Binding properties of Clostridium botulinum type C progenitor toxin to mucins., Nakamura T, Takada N, Tonozuka T, Sakano Y, Oguma K, Nishikawa A, Biochim Biophys Acta. 2007 Apr;1770(4):551-5. Epub 2006 Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17196748 17196748]
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Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells., Uotsu N, Nishikawa A, Watanabe T, Ohyama T, Tonozuka T, Sakano Y, Oguma K, Biochim Biophys Acta. 2006 Jan;1763(1):120-8. Epub 2005 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16413070 16413070]
[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Beta trefoil]]
[[Category: Beta trefoil]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:34:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:58:44 2008''

Revision as of 07:58, 28 July 2008

Image:2ehm.png

Template:STRUCTURE 2ehm

HA1 subcomponent of botulinum type C progenitor toxin complexed with N-acetylgalactosamine

Template:ABSTRACT PUBMED 18178224

About this Structure

2EHM is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.

Reference

Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:18178224

Binding properties of Clostridium botulinum type C progenitor toxin to mucins., Nakamura T, Takada N, Tonozuka T, Sakano Y, Oguma K, Nishikawa A, Biochim Biophys Acta. 2007 Apr;1770(4):551-5. Epub 2006 Nov 23. PMID:17196748

Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells., Uotsu N, Nishikawa A, Watanabe T, Ohyama T, Tonozuka T, Sakano Y, Oguma K, Biochim Biophys Acta. 2006 Jan;1763(1):120-8. Epub 2005 Dec 27. PMID:16413070

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