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1nzf

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[[Image:1nzf.gif|left|200px]]
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{{STRUCTURE_1nzf| PDB=1nzf | SCENE= }}
{{STRUCTURE_1nzf| PDB=1nzf | SCENE= }}
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'''T4 phage BGT-D100A mutant in complex with UDP-glucose: Form II'''
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===T4 phage BGT-D100A mutant in complex with UDP-glucose: Form II===
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==Overview==
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T4 phage beta-glucosyltransferase (BGT) is an inverting glycosyltransferase (GT) that transfers glucose from uridine diphospho-glucose (UDP-glucose) to an acceptor modified DNA. BGT belongs to the GT-B structural superfamily, represented, so far, by five different inverting or retaining GT families. Here, we report three high-resolution X-ray structures of BGT and a point mutant solved in the presence of UDP-glucose. The two co-crystal structures of the D100A mutant show that, unlike the wild-type enzyme, this mutation prevents glucose hydrolysis. This strongly indicates that Asp100 is the catalytic base. We obtained the wild-type BGT-UDP-glucose complex by soaking substrate-free BGT crystals. Comparison with a previous structure of BGT solved in the presence of the donor product UDP and an acceptor analogue provides the first model of an inverting GT-B enzyme in which both the donor and acceptor substrates are bound to the active site. The structural analyses support the in-line displacement reaction mechanism previously proposed, locate residues involved in donor substrate specificity and identify the catalytic base.
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The line below this paragraph, {{ABSTRACT_PUBMED_12860129}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12860129 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12860129}}
==About this Structure==
==About this Structure==
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[[Category: Gt-b]]
[[Category: Gt-b]]
[[Category: Udp-glucose]]
[[Category: Udp-glucose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:10:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:04:31 2008''

Revision as of 08:04, 28 July 2008

Image:1nzf.png

Template:STRUCTURE 1nzf

T4 phage BGT-D100A mutant in complex with UDP-glucose: Form II

Template:ABSTRACT PUBMED 12860129

About this Structure

1NZF is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: glucose binding and identification of the catalytic base for a direct displacement mechanism., Lariviere L, Gueguen-Chaignon V, Morera S, J Mol Biol. 2003 Jul 25;330(5):1077-86. PMID:12860129

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