2j5h

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{{STRUCTURE_2j5h| PDB=2j5h | SCENE= }}
{{STRUCTURE_2j5h| PDB=2j5h | SCENE= }}
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'''NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN'''
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===NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN===
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==Overview==
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We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition.
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{{ABSTRACT_PUBMED_17125258}}
==About this Structure==
==About this Structure==
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2J5H is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5H OCA].
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2J5H is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5H OCA].
==Reference==
==Reference==
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[[Category: Hormone/growth factor complex]]
[[Category: Hormone/growth factor complex]]
[[Category: Tumour progression]]
[[Category: Tumour progression]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:22:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:08:17 2008''

Revision as of 08:08, 28 July 2008

Image:2j5h.png

Template:STRUCTURE 2j5h

NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN

Template:ABSTRACT PUBMED 17125258

About this Structure

2J5H is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala., Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M, J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:17125258

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