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1ni7

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{{STRUCTURE_1ni7| PDB=1ni7 | SCENE= }}
{{STRUCTURE_1ni7| PDB=1ni7 | SCENE= }}
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'''NORTHEAST STRUCTURAL GENOMIC CONSORTIUM TARGET ER75'''
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===NORTHEAST STRUCTURAL GENOMIC CONSORTIUM TARGET ER75===
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==Overview==
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The structural biology of proteins mediating iron-sulfur (Fe-S) cluster assembly is central for understanding several important biological processes. Here we present the NMR structure of the 16-kDa protein YgdK from Escherichia coli, which shares 35% sequence identity with the E. coli protein SufE. The SufE X-ray crystal structure was solved in parallel with the YdgK NMR structure in the Northeast Structural Genomics (NESG) consortium. Both proteins are (1) key components for Fe-S metabolism, (2) exhibit the same distinct fold, and (3) belong to a family of at least 70 prokaryotic and eukaryotic sequence homologs. Accurate homology models were calculated for the YgdK/SufE family based on YgdK NMR and SufE crystal structure. Both structural templates contributed equally, exemplifying synergy of NMR and X-ray crystallography. SufE acts as an enhancer of the cysteine desulfurase activity of SufS by SufE-SufS complex formation. A homology model of CsdA, a desulfurase encoded in the same operon as YgdK, was modeled using the X-ray structure of SufS as a template. Protein surface and electrostatic complementarities strongly suggest that YgdK and CsdA likewise form a functional two-component desulfurase complex. Moreover, structural features of YgdK and SufS, which can be linked to their interaction with desulfurases, are conserved in all homology models. It thus appears very likely that all members of the YgdK/SufE family act as enhancers of Suf-S-like desulfurases. The present study exemplifies that "refined" selection of two (or more) targets enables high-quality homology modeling of large protein families.
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{{ABSTRACT_PUBMED_15930006}}
==About this Structure==
==About this Structure==
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1NI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NI7 OCA].
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1NI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NI7 OCA].
==Reference==
==Reference==
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[[Category: Rd-nmr]]
[[Category: Rd-nmr]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:11:00 2008''

Revision as of 08:11, 28 July 2008

Image:1ni7.png

Template:STRUCTURE 1ni7

NORTHEAST STRUCTURAL GENOMIC CONSORTIUM TARGET ER75

Template:ABSTRACT PUBMED 15930006

About this Structure

1NI7 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases., Liu G, Li Z, Chiang Y, Acton T, Montelione GT, Murray D, Szyperski T, Protein Sci. 2005 Jun;14(6):1597-608. PMID:15930006

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